Int. J. Mol. Sci. 2008, 9(12), 2366-2375; doi:10.3390/ijms9122366
Article

Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability

Received: 14 June 2008; in revised form: 13 November 2008 / Accepted: 25 November 2008 / Published: 27 November 2008
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Deoxynivalenol (DON) is one of the most common contaminants of various foodstuffs. A biotransformation system was used in order to lessen the toxicity of DON. A strain of Aspergillus (NJA-1) was isolated from soil and cultured in an inorganic salt medium containing DON. Bt2a/Bt2b primers were used to amplify the β-tubulin gene of NJA-1. Sequence analysis the PCR product and morphology observation indicated that NJA-1 belonged to Aspergillus tubingensis (aerobic fungi). The DNA sequence information of the PCR product was deposited in GenBank (accession number DQ9025790). The DNA sequence had 99% similarity to the Aspergillus tubingensis accession number AY820009. An unknown compound in NJA-1 showed the ability to convert DON into another product. The molecular weight of the bioconversion product was 18.1 D (H2O) larger than that of DON. The analysis showed that DON could be hydrolyzed by NJA-1. The mean DON biotransformation rate was 94.4% after two weeks of cultivation. The finding presents a new method for DON biotransformation.
Keywords: Trichothecene; Deoxynivalenol; Identification; Aspergillus tubingensis; Biotransformation
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MDPI and ACS Style

He, C.; Fan, Y.; Liu, G.; Zhang, H. Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability. Int. J. Mol. Sci. 2008, 9, 2366-2375.

AMA Style

He C, Fan Y, Liu G, Zhang H. Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability. International Journal of Molecular Sciences. 2008; 9(12):2366-2375.

Chicago/Turabian Style

He, Chenghua; Fan, Yanhong; Liu, Guofang; Zhang, Haibin. 2008. "Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability." Int. J. Mol. Sci. 9, no. 12: 2366-2375.

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