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Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China
* Author to whom correspondence should be addressed.
Received: 14 June 2008; in revised form: 13 November 2008 / Accepted: 25 November 2008 / Published: 27 November 2008
Abstract: Deoxynivalenol (DON) is one of the most common contaminants of various foodstuffs. A biotransformation system was used in order to lessen the toxicity of DON. A strain of Aspergillus (NJA-1) was isolated from soil and cultured in an inorganic salt medium containing DON. Bt2a/Bt2b primers were used to amplify the β-tubulin gene of NJA-1. Sequence analysis the PCR product and morphology observation indicated that NJA-1 belonged to Aspergillus tubingensis (aerobic fungi). The DNA sequence information of the PCR product was deposited in GenBank (accession number DQ9025790). The DNA sequence had 99% similarity to the Aspergillus tubingensis accession number AY820009. An unknown compound in NJA-1 showed the ability to convert DON into another product. The molecular weight of the bioconversion product was 18.1 D (H2O) larger than that of DON. The analysis showed that DON could be hydrolyzed by NJA-1. The mean DON biotransformation rate was 94.4% after two weeks of cultivation. The finding presents a new method for DON biotransformation.
Keywords: Trichothecene; Deoxynivalenol; Identification; Aspergillus tubingensis; Biotransformation
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MDPI and ACS Style
He, C.; Fan, Y.; Liu, G.; Zhang, H. Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability. Int. J. Mol. Sci. 2008, 9, 2366-2375.
He C, Fan Y, Liu G, Zhang H. Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability. International Journal of Molecular Sciences. 2008; 9(12):2366-2375.
He, Chenghua; Fan, Yanhong; Liu, Guofang; Zhang, Haibin. 2008. "Isolation and Identification of a Strain of Aspergillus Tubingensis With Deoxynivalenol Biotransformation Capability." Int. J. Mol. Sci. 9, no. 12: 2366-2375.