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Int. J. Mol. Sci. 2008, 9(11), 2265-2277; doi:10.3390/ijms9112265

Ponicidin Inhibits Monocytic Leukemia Cell Growth by Induction of Apoptosis

3,* , 1
Received: 23 September 2008 / Revised: 25 October 2008 / Accepted: 7 November 2008 / Published: 19 November 2008
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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In this study two monocytic leukemia cell lines, U937 and THP-1 cells, were used to investigate the anti-proliferation effects caused by ponicidin. Cell viability was measured by an MTT assay. Cell apoptosis was assessed by flow cytometry as well as DNA fragmentation analysis. Cell morphology was observed using an inverted microscope and Hoechst 33258 staining. RT-PCR and Western blot analysis were used to detect survivin as well as Bax and Bcl-2 expressions after the cells were treated with different concentrations of ponicidin. The results revealed that ponicidin could inhibit the growth of U937 and THP-1 cells significantly by induction of apoptosis. The suppression was in both time- and dose-dependent manner. Marked morphological changes of cell apoptosis were observed clearly after the cells were treated with ponicidin for 48~72 h. RT-PCR and Western blot analysis demonstrated that both survivin and Bcl-2 expressions were down-regulated remarkably while Bax expression remained constant before and after apoptosis occurred. We therefore conclude that ponicidin has significant anti-proliferation effects by inducing apoptosis on leukemia cells in vitro, downregulation of survivin as well as Bcl-2 expressions may be the important apoptosis inducing mechanisms. The results suggest that ponicidin may serve as potential therapeutic agent for leukemia.
Keywords: Ponicidin; Survivin; Bcl-2; Bax; Leukemia Ponicidin; Survivin; Bcl-2; Bax; Leukemia
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Liu, J.-J.; Zhang, Y.; Guang, W.-B.; Yang, H.-Z.; Lin, D.-J.; Xiao, R.-Z. Ponicidin Inhibits Monocytic Leukemia Cell Growth by Induction of Apoptosis. Int. J. Mol. Sci. 2008, 9, 2265-2277.

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