Int. J. Mol. Sci. 2008, 9(11), 2278-2289; doi:10.3390/ijms9112278
Article

A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector

Hongliang Huang 1 email, Hai Yu 2 email, Da Li 2 email, Yang Liu 2 email, Fenping Shen 2 email, Jun Zhou 3 email, Qingqing Wang 2,* email and Guping Tang 3,* email
1 School of Life Science, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P. R. China 2 Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China 3 Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Tianmushan Road 148, Hangzhou, P. R. China
* Authors to whom correspondence should be addressed.
Received: 31 May 2008; in revised form: 4 November 2008 / Accepted: 7 November 2008 / Published: 21 November 2008
(This article belongs to the Special Issue Functional Materials- From Functional Hybrid Materials to Functional Polymers)
PDF Full-text Download PDF Full-Text [578 KB, uploaded 21 November 2008 11:12 CET]
Abstract: A novel co-polymer based on 2-hydroxypropyl-α-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.
Keywords: Polyethylenimine; 2-hydroxypropyl-α-cyclodextrin; non-viral gene delivery vector; co-polymer

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Huang, H.; Yu, H.; Li, D.; Liu, Y.; Shen, F.; Zhou, J.; Wang, Q.; Tang, G. A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector. Int. J. Mol. Sci. 2008, 9, 2278-2289.

AMA Style

Huang H, Yu H, Li D, Liu Y, Shen F, Zhou J, Wang Q, Tang G. A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector. International Journal of Molecular Sciences. 2008; 9(11):2278-2289.

Chicago/Turabian Style

Huang, Hongliang; Yu, Hai; Li, Da; Liu, Yang; Shen, Fenping; Zhou, Jun; Wang, Qingqing; Tang, Guping. 2008. "A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector." Int. J. Mol. Sci. 9, no. 11: 2278-2289.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert