Protective Effect of Encapsulation in Fermentation of Limonene-contained Media and Orange Peel Hydrolyzate
Abstract
:1. Introduction
2. Results and Discussion
2.1. Enzymatic Hydrolysis
2.2. Free cell cultivation
2.3. Cultivation of encapsulated cells in the presence of limonene
2.4. Yeast cultivation in peel hydrolyzate
2.5. Selectivity of capsules’ membrane
2.6. Conclusions
3. Material and Methods
3.1. Substrates and enzymes
3.2. Hydrolyses
3.3. Yeast Strain and Media
3.4. Encapsulation Method and Batch Cultivation Experiments
3.5. Selectivity of capsules’ membrane
3.6. Analytical methods
Carbohydrate | % (of total solid) |
---|---|
Glucose | 22.9 ± 2.4 |
Fructose | 14.1 ± 1.3 |
Galactose | 4.0 ± 0.2 |
Arabinose | 7.1 ± 0.5 |
Xylose | 0.4 ± 0.1 |
Galacturonic acid | 19.0 ± 1.7 |
Total | 67.5 |
Parameter | Suspended Cells | Encapsulated cells with different limonene concentration (% v/v) of: | Peel hydrolyzate | |||
---|---|---|---|---|---|---|
0 | 0.5 | 1 | 1.5 | |||
−rS (g/L.h) | 4.0 | 8.56 | 5.96 | 5.38 | 5.58 | 5.00 |
rE(g/L.h) | 1.6 | 3.69 | 2.57 | 2.28 | 2.33 | 2.18 |
YSE(g/g) | 0.41(±0.03) | 0.43(±0.02) | 0.43(±0.01) | 0.42(±0.01) | 0.42(±0.01) | 0.44(±0.01) |
YSGly(g/g) | 0.080 | 0.062 | 0.050 | 0.048 | 0.047 | 0.050 |
YSAce(g/g) | 0.018 | 0.017 | 0.016 | 0.016 | 0.015 | 0.017 |
YSSuc(g/g) | 0.003 | 0.003 | 0.003 | 0.003 | 0.003 | 0.003 |
Acknowledgments
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Pourbafrani, M.; Talebnia, F.; Niklasson, C.; Taherzadeh, M.J. Protective Effect of Encapsulation in Fermentation of Limonene-contained Media and Orange Peel Hydrolyzate. Int. J. Mol. Sci. 2007, 8, 777-787. https://doi.org/10.3390/i8080777
Pourbafrani M, Talebnia F, Niklasson C, Taherzadeh MJ. Protective Effect of Encapsulation in Fermentation of Limonene-contained Media and Orange Peel Hydrolyzate. International Journal of Molecular Sciences. 2007; 8(8):777-787. https://doi.org/10.3390/i8080777
Chicago/Turabian StylePourbafrani, Mohammad, Farid Talebnia, Claes Niklasson, and Mohammad J. Taherzadeh. 2007. "Protective Effect of Encapsulation in Fermentation of Limonene-contained Media and Orange Peel Hydrolyzate" International Journal of Molecular Sciences 8, no. 8: 777-787. https://doi.org/10.3390/i8080777