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Int. J. Mol. Sci. 2007, 8(7), 583-592; doi:10.3390/i8070583
Article

Drastic Attenuation of Pseudomonas aeruginosa Pathogenicity in a Holoxenic Mouse Experimental Model Induced by Subinhibitory Concentrations of Phenyllactic acid (PLA)

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Received: 29 January 2007; in revised form: 25 June 2007 / Accepted: 26 June 2007 / Published: 2 July 2007
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: The discovery of communication systems regulating bacterial virulence hasafforded a novel opportunity to control infectious bacteria without interfering withgrowth. In this paper we describe the effect of subinhibitory concentrations of phenyl-lactic acid (PLA) on the pathogenicity of Pseudomonas aeruginosa in mice. The animalswere inoculated by oral (p.o.), intranasal (i.n.), intravenous (i.v.) and intraperitoneal (i.p.)routes with P. aeruginoasa wild and PLA-treated cultures. The mice were followed upduring 16 days after infection and the body weight, mortality and morbidity rate weremeasured every day. The microbial charge was studied by viable cell counts in lungs,spleen, intestinal mucosa and blood. The mice batches infected with wild P. aeruginosabacterial cultures exhibited high mortality rates (100 % after i.v. and i.p. route) and veryhigh cell counts in blood, lungs, intestine and spleen. In contrast, the animal batchesinfected with PLA treated bacterial cultures exhibited good survival rates (0 % mortality) and the viable cell counts in the internal organs revealed with one exception the complete abolition of the invasive capacity of the tested strains. In this study, using a mouse infection model we show that D-3-phenyllactic acid (PLA) can act as a potent antagonist of Pseudomonas (P.) aeruginosa pathogenicity, without interfering with the bacterial growth, as demonstrated by the improvement of the survival rates as well as the clearance of bacterial strains from the body.
Keywords: Phenyllactic acid; Pseudomonas aeruginosa; in vivo holoxenic mouse model; virulence attenuation Phenyllactic acid; Pseudomonas aeruginosa; in vivo holoxenic mouse model; virulence attenuation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chifiriuc, M.; Veronica, L.; Dracea, O.; Ditu, L.-M.; Smarandache, D.; Bucur, M.; Larion, C.; Ramona, C.; Sasarman, E. Drastic Attenuation of Pseudomonas aeruginosa Pathogenicity in a Holoxenic Mouse Experimental Model Induced by Subinhibitory Concentrations of Phenyllactic acid (PLA). Int. J. Mol. Sci. 2007, 8, 583-592.

AMA Style

Chifiriuc M, Veronica L, Dracea O, Ditu L-M, Smarandache D, Bucur M, Larion C, Ramona C, Sasarman E. Drastic Attenuation of Pseudomonas aeruginosa Pathogenicity in a Holoxenic Mouse Experimental Model Induced by Subinhibitory Concentrations of Phenyllactic acid (PLA). International Journal of Molecular Sciences. 2007; 8(7):583-592.

Chicago/Turabian Style

Chifiriuc, Mariana–Carmen; Veronica, Lazar; Dracea, Olguta; Ditu, Lia-Mara; Smarandache, Diana; Bucur, Marcela; Larion, Cristina; Ramona, Cernat; Sasarman, Elena. 2007. "Drastic Attenuation of Pseudomonas aeruginosa Pathogenicity in a Holoxenic Mouse Experimental Model Induced by Subinhibitory Concentrations of Phenyllactic acid (PLA)." Int. J. Mol. Sci. 8, no. 7: 583-592.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert