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Int. J. Mol. Sci. 2007, 8(5), 455-469; doi:10.3390/i8050455
Article

Polarization of T Lymphocytes Is Regulated by Mesenchymal Stem Cells in NZBWF1 and BALB/c Mice

1,* , 1
,
2
 and
3
1 Department of Immunology and Rheumatology, Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, P R China 2 Department of Hematology, Subei People’s Hospital of Jiangsu Province, Yangzhou 225001, P R China 3 Immunology and Reproductive Biology Lab, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, P R China
* Author to whom correspondence should be addressed.
Received: 27 February 2007 / Revised: 17 April 2007 / Accepted: 9 May 2007 / Published: 30 May 2007
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Mesenchymal stem cells (MSCs) have been shown to suppress proliferation andactivation of T lymphocytes in vivo and in vitro although the molecular mechanism of theimmunosuppressive effect is not completely understood. To investigate theimmunoregulatory effects of mice bone marrow mesenchymal stem cells on T lymphocyte,MSCs from NZBWF1 and BALB/c mice were isolated and expanded from bone marrow,and identified with cell morphology and the surface phenotypes. CD3+ T lymphocytesisolated by nylon wool columns were co-cultured with PMA with or without the two strainsof MSCs. Then T cell apoptosis and intercellular cytokines of T cell were assessed by flowcytometry. Quantification of transcription factors T-box (T-bet) and GATA-binding protein3 (GATA-3) expressed in T cells was detected by RT-PCR and western blot. Our resultsshowed that there was a decrease of CD3+ T cell apoptosis when NW MSCs or Bc MSCswere added, and an increase of Th2 subset by NW MSCs and Th1 subset by Bc MSCs wereobserved by co-culturing MSCs with T lymphocytes. It is suggested that, by favoring Th1-cell development and inhibitory Th2-cell development, normal MSCs might interfere withthe SLE development, and that marrow-derived NW MSCs had defectiveimmunoregulatory function when compared with MSCs from healthy mouse strains.
Keywords: NZBWF1 mice; BALB/c mice; mesenchymal stem cells (MSCs); systemic lupus erythematosus (SLE); T lymphocytes; Th1/Th2 NZBWF1 mice; BALB/c mice; mesenchymal stem cells (MSCs); systemic lupus erythematosus (SLE); T lymphocytes; Th1/Th2
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Sun, L.; Liang, J.; Li, H.; Hou, Y. Polarization of T Lymphocytes Is Regulated by Mesenchymal Stem Cells in NZBWF1 and BALB/c Mice. Int. J. Mol. Sci. 2007, 8, 455-469.

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