Int. J. Mol. Sci. 2007, 8(5), 455-469; doi:10.3390/i8050455
Article

Polarization of T Lymphocytes Is Regulated by Mesenchymal Stem Cells in NZBWF1 and BALB/c Mice

1,* email, 1email, 2email and 3email
Received: 27 February 2007; in revised form: 17 April 2007 / Accepted: 9 May 2007 / Published: 30 May 2007
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Mesenchymal stem cells (MSCs) have been shown to suppress proliferation andactivation of T lymphocytes in vivo and in vitro although the molecular mechanism of theimmunosuppressive effect is not completely understood. To investigate theimmunoregulatory effects of mice bone marrow mesenchymal stem cells on T lymphocyte,MSCs from NZBWF1 and BALB/c mice were isolated and expanded from bone marrow,and identified with cell morphology and the surface phenotypes. CD3+ T lymphocytesisolated by nylon wool columns were co-cultured with PMA with or without the two strainsof MSCs. Then T cell apoptosis and intercellular cytokines of T cell were assessed by flowcytometry. Quantification of transcription factors T-box (T-bet) and GATA-binding protein3 (GATA-3) expressed in T cells was detected by RT-PCR and western blot. Our resultsshowed that there was a decrease of CD3+ T cell apoptosis when NW MSCs or Bc MSCswere added, and an increase of Th2 subset by NW MSCs and Th1 subset by Bc MSCs wereobserved by co-culturing MSCs with T lymphocytes. It is suggested that, by favoring Th1-cell development and inhibitory Th2-cell development, normal MSCs might interfere withthe SLE development, and that marrow-derived NW MSCs had defectiveimmunoregulatory function when compared with MSCs from healthy mouse strains.
Keywords: NZBWF1 mice; BALB/c mice; mesenchymal stem cells (MSCs); systemic lupus erythematosus (SLE); T lymphocytes; Th1/Th2
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MDPI and ACS Style

Sun, L.; Liang, J.; Li, H.; Hou, Y. Polarization of T Lymphocytes Is Regulated by Mesenchymal Stem Cells in NZBWF1 and BALB/c Mice. Int. J. Mol. Sci. 2007, 8, 455-469.

AMA Style

Sun L, Liang J, Li H, Hou Y. Polarization of T Lymphocytes Is Regulated by Mesenchymal Stem Cells in NZBWF1 and BALB/c Mice. International Journal of Molecular Sciences. 2007; 8(5):455-469.

Chicago/Turabian Style

Sun, Lingyun; Liang, Jun; Li, Hui; Hou, Yayi. 2007. "Polarization of T Lymphocytes Is Regulated by Mesenchymal Stem Cells in NZBWF1 and BALB/c Mice." Int. J. Mol. Sci. 8, no. 5: 455-469.


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