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Int. J. Mol. Sci. 2007, 8(5), 445-454; doi:10.3390/i8050445
Article

Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody

1,* , 2, 1, 2
, 3
 and 4,*
Received: 10 February 2007; in revised form: 12 April 2007 / Accepted: 16 May 2006 / Published: 29 May 2007
(This article belongs to the Section Molecular Recognition)
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Abstract: The small molecule, meso-tetra(α,α,α,α-o-phenylacetamidophenyl)porphyrin(Mr1147.0) was used as complete antigen to elicit MAb through the immunization and cellfusion techniques.The MAb 1F2 obtained was demonstrated to be very pure byMALDI/TOFMS.The subtype of MAb 1F2 is IgG2a, which has a relative molecular weightof 156,678.8 Da.No significant change in the intensity of absorption peaks in UV and CDspectra was observed over a pH range between 6 and 12.The high stability of the abzymeand the tight binding between Fe porphyrin and antibody were also demonstrated.Vmax, Km,κcat, κcat/Km for abzyme are 5.18 × 10-8 Ms-1, 1.50 × 10-8M , 0.518 s-1, 3.45 × 107 M-1s-1, respectively.The data obtained indicate that catalytic antibody has highcatalytic activity.The chloroperoxidase activity of MAb 1F2-Fe porphyrin complex is stablefrom 10 °C to 60 °C.
Keywords: meso-Tetra(α,α,α,α-o-phenylacetamidophenyl)porphyrin; MAb; Complete antigen; Chloroperoxidase; Abzyme meso-Tetra(α,α,α,α-o-phenylacetamidophenyl)porphyrin; MAb; Complete antigen; Chloroperoxidase; Abzyme
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, F.; Huang, X.; Du, L.; Li, W.; He, H.; Qi, C. Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody. Int. J. Mol. Sci. 2007, 8, 445-454.

AMA Style

Wang F, Huang X, Du L, Li W, He H, Qi C. Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody. International Journal of Molecular Sciences. 2007; 8(5):445-454.

Chicago/Turabian Style

Wang, Fengyang; Huang, Xueying; Du, Li; Li, Weiguo; He, Hongxuan; Qi, Chao. 2007. "Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody." Int. J. Mol. Sci. 8, no. 5: 445-454.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert