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Int. J. Mol. Sci. 2007, 8(5), 433-444; doi:10.3390/i8050433
Article

Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway

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1 State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, 200433, People’s Republic of China 2 Department of C&M Physiology Penn State College of Medicine Hershey, PA17033, USA
* Authors to whom correspondence should be addressed.
Received: 18 April 2007 / Revised: 5 May 2007 / Accepted: 10 May 2007 / Published: 25 May 2007
(This article belongs to the Special Issue Interaction of Biological Molecules)
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Abstract

The glycine-N-acyltransferase (GLYAT) is well known to be involved in thedetoxification of endogenous and exogenous xenobiotic acyl-CoA's in mammals.Unfortunately, the knowledge about the gene encoding GLYAT is very limited. Here wereport a novel gene encoding a GLYAT member, designated as GLYATL1, which was1546 base pairs in length and contained an open reading frame (ORF) encoding apolypeptide of 302 amino acids. GLYATL1 was a split gene that was consisted of 7 exonsand 6 introns and mapped to chromosome 11q12.1. The expression of GLYATL1 could befound in liver, kidney, pancreas, testis, ovary and stomach among 18 human tissues by RT-PCR analysis. Subcellular localization of myc-tagged GLYATL1 fusion protein revealedthat GLYATL1 was distributed primarily in the cytoplasm of COS-7 cells. Furthermore,through the pathway profiling assay, the GLYATL1 protein was found to activate HSEsignaling pathway in a dose-dependent manner when overexpressed in HEK293T cells.
Keywords: GLYATL1; HSE pathway; Glycine-N-acyltransferase; GLYATL1; HSE pathway; Glycine-N-acyltransferase;
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Zhang, H.; Lang, Q.; Li, J.; Zhong, Z.; Xie, F.; Ye, G.; Wan, B.; Yu, L. Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway. Int. J. Mol. Sci. 2007, 8, 433-444.

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