Int. J. Mol. Sci. 2007, 8(5), 433-444; doi:10.3390/i8050433
Article

Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway

1 State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, 200433, People’s Republic of China 2 Department of C&M Physiology Penn State College of Medicine Hershey, PA17033, USA
* Authors to whom correspondence should be addressed.
Received: 18 April 2007; in revised form: 5 May 2007 / Accepted: 10 May 2007 / Published: 25 May 2007
(This article belongs to the Special Issue Interaction of Biological Molecules)
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Abstract: The glycine-N-acyltransferase (GLYAT) is well known to be involved in thedetoxification of endogenous and exogenous xenobiotic acyl-CoA's in mammals.Unfortunately, the knowledge about the gene encoding GLYAT is very limited. Here wereport a novel gene encoding a GLYAT member, designated as GLYATL1, which was1546 base pairs in length and contained an open reading frame (ORF) encoding apolypeptide of 302 amino acids. GLYATL1 was a split gene that was consisted of 7 exonsand 6 introns and mapped to chromosome 11q12.1. The expression of GLYATL1 could befound in liver, kidney, pancreas, testis, ovary and stomach among 18 human tissues by RT-PCR analysis. Subcellular localization of myc-tagged GLYATL1 fusion protein revealedthat GLYATL1 was distributed primarily in the cytoplasm of COS-7 cells. Furthermore,through the pathway profiling assay, the GLYATL1 protein was found to activate HSEsignaling pathway in a dose-dependent manner when overexpressed in HEK293T cells.
Keywords: GLYATL1; HSE pathway; Glycine-N-acyltransferase;

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MDPI and ACS Style

Zhang, H.; Lang, Q.; Li, J.; Zhong, Z.; Xie, F.; Ye, G.; Wan, B.; Yu, L. Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway. Int. J. Mol. Sci. 2007, 8, 433-444.

AMA Style

Zhang H, Lang Q, Li J, Zhong Z, Xie F, Ye G, Wan B, Yu L. Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway. International Journal of Molecular Sciences. 2007; 8(5):433-444.

Chicago/Turabian Style

Zhang, Haoxing; Lang, Qingyu; Li, Jie; Zhong, Zhaomin; Xie, Fang; Ye, Guangming; Wan, Bo; Yu, Long. 2007. "Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway." Int. J. Mol. Sci. 8, no. 5: 433-444.

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