Int. J. Mol. Sci. 2007, 8(5), 433-444; doi:10.3390/i8050433
Article

Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway

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Received: 18 April 2007; in revised form: 5 May 2007 / Accepted: 10 May 2007 / Published: 25 May 2007
(This article belongs to the Special Issue Interaction of Biological Molecules)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The glycine-N-acyltransferase (GLYAT) is well known to be involved in thedetoxification of endogenous and exogenous xenobiotic acyl-CoA's in mammals.Unfortunately, the knowledge about the gene encoding GLYAT is very limited. Here wereport a novel gene encoding a GLYAT member, designated as GLYATL1, which was1546 base pairs in length and contained an open reading frame (ORF) encoding apolypeptide of 302 amino acids. GLYATL1 was a split gene that was consisted of 7 exonsand 6 introns and mapped to chromosome 11q12.1. The expression of GLYATL1 could befound in liver, kidney, pancreas, testis, ovary and stomach among 18 human tissues by RT-PCR analysis. Subcellular localization of myc-tagged GLYATL1 fusion protein revealedthat GLYATL1 was distributed primarily in the cytoplasm of COS-7 cells. Furthermore,through the pathway profiling assay, the GLYATL1 protein was found to activate HSEsignaling pathway in a dose-dependent manner when overexpressed in HEK293T cells.
Keywords: GLYATL1; HSE pathway; Glycine-N-acyltransferase;
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MDPI and ACS Style

Zhang, H.; Lang, Q.; Li, J.; Zhong, Z.; Xie, F.; Ye, G.; Wan, B.; Yu, L. Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway. Int. J. Mol. Sci. 2007, 8, 433-444.

AMA Style

Zhang H, Lang Q, Li J, Zhong Z, Xie F, Ye G, Wan B, Yu L. Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway. International Journal of Molecular Sciences. 2007; 8(5):433-444.

Chicago/Turabian Style

Zhang, Haoxing; Lang, Qingyu; Li, Jie; Zhong, Zhaomin; Xie, Fang; Ye, Guangming; Wan, Bo; Yu, Long. 2007. "Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway." Int. J. Mol. Sci. 8, no. 5: 433-444.

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