Next Article in Journal
Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody
Next Article in Special Issue
High-level Expression of Cecropin X in Escherichia coli
Previous Article in Journal
Prediction of Standard Enthalpy of Formation by a QSPR Model
Previous Article in Special Issue
How Good Can the Characteristic Polynomial Be for Correlations?
Int. J. Mol. Sci. 2007, 8(5), 433-444; doi:10.3390/i8050433

Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway

1,*  and 1,*
1 State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, 200433, People’s Republic of China 2 Department of C&M Physiology Penn State College of Medicine Hershey, PA17033, USA
* Authors to whom correspondence should be addressed.
Received: 18 April 2007 / Revised: 5 May 2007 / Accepted: 10 May 2007 / Published: 25 May 2007
(This article belongs to the Special Issue Interaction of Biological Molecules)
View Full-Text   |   Download PDF [1681 KB, uploaded 19 June 2014]   |   Browse Figures


The glycine-N-acyltransferase (GLYAT) is well known to be involved in thedetoxification of endogenous and exogenous xenobiotic acyl-CoA's in mammals.Unfortunately, the knowledge about the gene encoding GLYAT is very limited. Here wereport a novel gene encoding a GLYAT member, designated as GLYATL1, which was1546 base pairs in length and contained an open reading frame (ORF) encoding apolypeptide of 302 amino acids. GLYATL1 was a split gene that was consisted of 7 exonsand 6 introns and mapped to chromosome 11q12.1. The expression of GLYATL1 could befound in liver, kidney, pancreas, testis, ovary and stomach among 18 human tissues by RT-PCR analysis. Subcellular localization of myc-tagged GLYATL1 fusion protein revealedthat GLYATL1 was distributed primarily in the cytoplasm of COS-7 cells. Furthermore,through the pathway profiling assay, the GLYATL1 protein was found to activate HSEsignaling pathway in a dose-dependent manner when overexpressed in HEK293T cells.
Keywords: GLYATL1; HSE pathway; Glycine-N-acyltransferase; GLYATL1; HSE pathway; Glycine-N-acyltransferase;
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Zhang, H.; Lang, Q.; Li, J.; Zhong, Z.; Xie, F.; Ye, G.; Wan, B.; Yu, L. Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway. Int. J. Mol. Sci. 2007, 8, 433-444.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert