Localization Microscopy of Actin Cytoskeleton in Human Platelets
AbstractHere, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm. View Full-Text
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Mayr, S.; Hauser, F.; Peterbauer, A.; Tauscher, A.; Naderer, C.; Axmann, M.; Plochberger, B.; Jacak, J. Localization Microscopy of Actin Cytoskeleton in Human Platelets. Int. J. Mol. Sci. 2018, 19, 1150.
Mayr S, Hauser F, Peterbauer A, Tauscher A, Naderer C, Axmann M, Plochberger B, Jacak J. Localization Microscopy of Actin Cytoskeleton in Human Platelets. International Journal of Molecular Sciences. 2018; 19(4):1150.Chicago/Turabian Style
Mayr, Sandra; Hauser, Fabian; Peterbauer, Anja; Tauscher, Andreas; Naderer, Christoph; Axmann, Markus; Plochberger, Birgit; Jacak, Jaroslaw. 2018. "Localization Microscopy of Actin Cytoskeleton in Human Platelets." Int. J. Mol. Sci. 19, no. 4: 1150.
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