Special Issue "Single Cell Technology"
Deadline for manuscript submissions: 20 October 2018
Prof. Fan-Gang Tseng
Department of Engineering and System Science, Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu, Taiwan, ROC-30013
Website | E-Mail
Interests: MEMS/NEMS; microfluidics; 3D tissue culture; electrochemical sensors; nanotechnology in biomedical applications
The cell is the most fundamental building block in our body and it is presently a great challenge in cell biology to understand cell-to-cell responses, and how an individual cell delivers its information to others. The average ensemble measurement of cells cannot provide information regarding the detailed behaviors of any individual cell; however, each cell in heterogeneous populations has its own unique behavior and it has different responses, even after treatment with the same reagents or drugs as other cells. On the other hand, single cell sequencing (SCS) is able to empirically infer driver mutations and to map sequential mutation events during cancer development. The integration of single-cell genomics and transcriptomics is able to provide functional consequences of mutations and the copy number variations of cells. Thus, single cell technology (SCT) has the power to understand fundamental cell biology in embryonic development, detailed knowledge of cell lineage trees in higher organisms, to dissect tumor heterogeneity and disease, etc. Recently, the development of micro/nanofluidic technologies have enabled the characterization of a single cell in the micro/nanoscale environments with a massively parallel platform, which can distinguish cell-to-cell heterogeneity via tracking responses over time. The devices are, not only useful for cell manipulation, isolation, separation, and analysis, but are also able to control cellular parameters at the single cell level.
This Special Issue will invite the latest research articles and reviews dealing with SCT, and the role of SCT in omics. Applications and future prospects, with their advantages and limitations, are also welcome.
Dr. Tuhin Subhra Santra
Prof. Dr. Fan-Gang Tseng
Manuscript Submission Information
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- Single cells interaction, perturbation, cultivation, cellular heterogeneity
- single cell in system biology
- single cell cytometry
- micro/nano fluidic devices for single cell analysis
- single cell manipulation, separation, detection
- single cell genomics, proteomics, transcriptomics, metabolomics and fluxomics
- single cell diagnostics and imaging
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Authors: Daniel Andersson 1, David Svec 1,2, Cathrine Pedersen 3, Jørn Remi Henriksen 3, and Anders Ståhlberg 1,4,5*.
1 Sahlgrenska Cancer Center, Department of Pathology and Genetics, Sahlgrenska Academy at University of Gothenburg, Box 425, 40530 Gothenburg, Sweden.
2 Institute of Biotechnology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague 4, 14221, Czech Republic.
3 ArcticZymes AS, Sykehusveien 23, 9019 Tromsø, Norway.
4 Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden.
5 Department of Clinical Pathology and Genetics, Sahlgrenska University Hospital, 413 45 Gothenburg, Sweden.
*corresponding author: Anders Ståhlberg, Sahlgrenska Cancer Center, University of Gothenburg, Box 425, 405 30 Gothenburg, Sweden; Tel: +46 31 7866735; email@example.com
Abstract: Analysis of rare DNA and RNA molecules in samples of limited sizes, such as liquid biopsies and single cells, often requires preamplification, making downstream analyses particularly sensitive to PCR generated contamination. To address this issue, we here assessed the feasibility of performing cod uracil-DNA N-glycosylase (Cod UNG) treatment in combination with targeted preamplification using dUTP to eliminate carry-over DNA. Cod UNG can be completely and irreversibly heat inactivated, a prerequisite in applications involving preamplification, where any loss of amplicons is detrimental to subsequent quantification. Using 96 target assays, we show using quantitative real-time PCR (qPCR) that replacement of dTTP with dUTP in the preamplification reaction mix yield comparable dynamic range, reproducibility and sensitivity. Moreover, Cod UNG essentially removes all uracil-containing template of most assays, regardless of initial concentration, without affecting downstream analyses. Finally, we demonstrate the use of Cod UNG and dUTP in targeted preamplification can easily be included in the workflow for single-cell gene expression profiling. In summary, Cod UNG treatment in combination with targeted preamplification using dUTP offers a simple and efficient solution to prevent carry-over contamination and the generation of false positives and erroneous quantification.