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Int. J. Mol. Sci. 2018, 19(2), 501; https://doi.org/10.3390/ijms19020501

MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat

1
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
2
Sichuan Province General Station of Animal Husbandry, Chengdu 611130, China
3
College of Life and Science, Sichuan Agricultural University, Chengdu 611130, China
4
Chongqing Academy of Animal Sciences, Chongqing 402460, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 18 December 2017 / Revised: 18 January 2018 / Accepted: 25 January 2018 / Published: 7 February 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Intramuscular fat (IMF) content and composition are considered crucial indicators of porcine meat quality. However, the molecular mechanism of porcine IMF development is still mostly unclear. Recently, new evidence suggested that microRNA (miRNAs) play important roles in porcine intramuscular adipogenesis. Previously, microRNA-125a-5p (miR-125a-5p) was identified as an important regulator of adipogenesis. In the present study, we found that the expression of miR-125a-5p is dynamically regulated during porcine intramuscular preadipocytes differentiation and that its expression levels in different porcine muscle tissues were negatively involved with IMF content. To investigate the potential function role of miR-125a-5p in IMF development, porcine intramuscular preadipocytes were collected and transfected with miR-125a-5p mimics, inhibitors, or a negative control (NC), respectively. The results showed that overexpression of miR-125a-5p promoted proliferation and inhibited differentiation of porcine intramuscular preadipocytes while inhibition of miR-125a-5p had the opposite effects. Furthermore, a luciferase reporter assay demonstrated that porcine kruppel like factor 3 (KLF13) is a target gene of miR-125a-5p during porcine intramuscular preadipocytes differentiation. Interestingly, porcine ELOVL fatty acid elongase 6 (ELOVL6), a regulator of fatty acid composition, was also identified as a target gene of miR-125a-5p during porcine intramuscular adipogenesis. Further studies show that miR-125a-5p overexpression reduced total saturated fatty acids (SFA) content and monounsaturated fatty acids (MUFA)/SFA ratios while having no significant impact on polyunsaturated fatty acids (PUFA)/SFA and n-6/n-3 ratios. Taken together, our results identified that miR-125a-5p may be a novel regulator of porcine intramuscular adipogenesis and the fatty acid composition of porcine IMF. View Full-Text
Keywords: intramuscular fat; fatty acid composition; differentiation; micoRNA-125a-5p; kruppel like factor 3 intramuscular fat; fatty acid composition; differentiation; micoRNA-125a-5p; kruppel like factor 3
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Du, J.; Xu, Y.; Zhang, P.; Zhao, X.; Gan, M.; Li, Q.; Ma, J.; Tang, G.; Jiang, Y.; Wang, J.; Li, X.; Zhang, S.; Zhu, L. MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat. Int. J. Mol. Sci. 2018, 19, 501.

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