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Int. J. Mol. Sci. 2017, 18(8), 1728; doi:10.3390/ijms18081728

Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors

Dpto. de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain
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Received: 17 July 2017 / Revised: 4 August 2017 / Accepted: 4 August 2017 / Published: 8 August 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Control and quantification of effector molecules such as heavy metals, toxins or other target molecules is of great biotechnological, social and economic interest. Microorganisms have regulatory proteins that recognize and modify the gene expression in the presence or absence of these compounds (effector molecules) by means of binding to gene sequences. The association of these recognizing gene sequences to reporter genes will allow the detection of effector molecules of interest with high sensitivity. Once investigators have these two elements—recognizing gene sequences and reporter genes that emit signals—we need a suitable vehicle to introduce both elements. Here, we suggest lactic acid bacteria (LAB) and bifidobacteria as promising carrier microorganisms for these molecular biosensors. The use of fluorescent proteins as well as food-grade vectors and clustered regularly interspaced short palindromic repeats (CRISPR) are indispensable tools for introducing biosensors into these microorganisms. The use of these LAB and bifidobacteria would be of special interest for studying the intestinal environment or other complex ecosystems. The great variety of species adapted to many environments, as well as the possibility of applying several protocols for their transformation with recognizing gene sequences and reporter genes are considerable advantages. Finally, an effort must be made to find recognizable gene sequences. View Full-Text
Keywords: DNA microbial biosensors; lactic acid bacteria; bifidobacteria; fluorescent proteins; cloning vectors; clustered regularly interspaced short palindromic repeats DNA microbial biosensors; lactic acid bacteria; bifidobacteria; fluorescent proteins; cloning vectors; clustered regularly interspaced short palindromic repeats
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Landete, J.M.; Arqués, J.L. Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors. Int. J. Mol. Sci. 2017, 18, 1728.

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