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Int. J. Mol. Sci. 2017, 18(8), 1725; doi:10.3390/ijms18081725

The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis

Department of Prosthodontics, Dental Research Institute, Institute of Translational Dental Sciences, BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan 50612, Korea
Department of CognoMechatronics Engineering, Pusan National University, Busan 46241, Korea
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 18 July 2017 / Revised: 31 July 2017 / Accepted: 6 August 2017 / Published: 8 August 2017
(This article belongs to the Special Issue Nano/Micro-Assisted Regenerative Medicine)
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This study was conducted to evaluate the effect of biphasic calcium phosphate (BCP) coated with reduced graphene oxide (rGO) as bone graft materials on bone regeneration. The rGO-coated BCP bone graft material was fabricatied by mixing rGO and BCP at various concentrations. The surface charge of rGO-coated BCP was measured to be −14.43 mV, which formed a static electrostatic interaction. Cell viabilities were significantly diminished at higher concentrations of ≥100 μg/mL. The calvarial defects of 48 rats were implanted rGO-coated BCPs at a weight ratio of 2:1000 (rGO2), 4:1000 (rGO4), and 10:1000 (rGO10), repectively. BCP was used as a control group. The micro-CT and histological analysis were performed to evaluate new bone formation at 2 and 8 weeks after surgery. The results showed that the new bone volume (mm3) was significantly higher in the experimental groups than in the control group. Histological analysis showed that new bone areas (%) were significantly higher in the rGO2 and rGO10 than in the control, and significantly higher in rGO4 than in the rGO2 and rGO10. Conclusively, the rGO-coated BCP was found to be effective on osteogenesis and the concentration of the composite was an important factor. View Full-Text
Keywords: reduced graphene oxide; biphasic calcium phosphate; bone regeneration; concentration; rat reduced graphene oxide; biphasic calcium phosphate; bone regeneration; concentration; rat

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kim, J.-W.; Shin, Y.C.; Lee, J.-J.; Bae, E.-B.; Jeon, Y.-C.; Jeong, C.-M.; Yun, M.-J.; Lee, S.-H.; Han, D.-W.; Huh, J.-B. The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis. Int. J. Mol. Sci. 2017, 18, 1725.

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