A Simple Procedure for the Evaluation of Bone Vitality by Staining with a Tetrazolium Salt
AbstractPresently, no intra-operative method for a direct assessment of bone vitality exists. Therefore, we set out to test the applicability of tetrazolium-based staining on bone samples. The explanted femoral heads of 37 patients were used to obtain either cancellous bone fragments or bone slices. Samples were stained with 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) or 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (thiazolyl blue, MTT) at different times (one to twelve hours) after explantation. Staining was quantified either spectrophotometrically after extraction of the dyes or by densitometric image analysis. TTC-staining of cancellous bone fragments and bone slices, respectively, indicated the detectability of vital cells in both types of samples in a window of up to six hours after explantation. Staining intensity at later time-points was indistinguishable from the staining of untreated samples or sodium azide treated samples, which represent dead cells. In contrast, MTT-staining of bone slices revealed intense unspecific staining, which obscured the evaluation of the vitality of the samples. The lack of a detectable increase of colour intensity in TTC-stained bone samples, which were treated more than six hours after explantation, corresponds to reduced fracture healing. The described simple procedure could provide a basis for an intraoperative decision by the orthopaedic surgeon. View Full-Text
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Schiffner, R.; Reiche, J.; Brodt, S.; Brinkmann, O.; Bungartz, M.; Matziolis, G.; Schmidt, M. A Simple Procedure for the Evaluation of Bone Vitality by Staining with a Tetrazolium Salt. Int. J. Mol. Sci. 2017, 18, 1646.
Schiffner R, Reiche J, Brodt S, Brinkmann O, Bungartz M, Matziolis G, Schmidt M. A Simple Procedure for the Evaluation of Bone Vitality by Staining with a Tetrazolium Salt. International Journal of Molecular Sciences. 2017; 18(8):1646.Chicago/Turabian Style
Schiffner, René; Reiche, Juliane; Brodt, Steffen; Brinkmann, Olaf; Bungartz, Matthias; Matziolis, Georg; Schmidt, Martin. 2017. "A Simple Procedure for the Evaluation of Bone Vitality by Staining with a Tetrazolium Salt." Int. J. Mol. Sci. 18, no. 8: 1646.
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