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Int. J. Mol. Sci. 2017, 18(6), 1235; doi:10.3390/ijms18061235

Micro-Electromechanical Affinity Sensor for the Monitoring of Glucose in Bioprocess Media

1
Chair of Bioprocess Engineering, Department of Biotechnology, Technical University Berlin, ACK24, Ackerstr. 76, 13355 Berlin, Germany
2
IHP, Im Technologiepark 25, 15236 Frankfurt (Oder), Germany
Present address: RISE Acreo, Box 787, SE-60117 Norrköping, Sweden.
*
Author to whom correspondence should be addressed.
Academic Editor: Seiya Tsujimura
Received: 8 February 2017 / Revised: 23 April 2017 / Accepted: 31 May 2017 / Published: 8 June 2017
(This article belongs to the Special Issue Biomolecular Engineering and Bioelectronics)
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Abstract

An affinity-viscometry-based micro-sensor probe for continuous glucose monitoring was investigated with respect to its suitability for bioprocesses. The sensor operates with glucose and dextran competing as binding partner for concanavalin A, while the viscosity of the assay scales with glucose concentration. Changes in viscosity are determined with a micro-electromechanical system (MEMS) in the measurement cavity of the sensor probe. The study aimed to elucidate the interactions between the assay and a typical phosphate buffered bacterial cultivation medium. It turned out that contact with the medium resulted in a significant long-lasting drift of the assay’s viscosity at zero glucose concentration. Adding glucose to the medium lowers the drift by a factor of eight. The cglc values measured off-line with the glucose sensor for monitoring of a bacterial cultivation were similar to the measurements with an enzymatic assay with a difference of less than ±0.15 g·L−1. We propose that lectin agglomeration, the electro-viscous effect, and constitutional changes of concanavalin A due to exchanges of the incorporated metal ions may account for the observed viscosity increase. The study has demonstrated the potential of the MEMS sensor to determine sensitive viscosity changes within very small sample volumes, which could be of interest for various biotechnological applications. View Full-Text
Keywords: bioprocess; affinity assay; viscometer; glucose monitoring; concanavalin A; bacterial culture media bioprocess; affinity assay; viscometer; glucose monitoring; concanavalin A; bacterial culture media
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MDPI and ACS Style

Theuer, L.; Lehmann, M.; Junne, S.; Neubauer, P.; Birkholz, M. Micro-Electromechanical Affinity Sensor for the Monitoring of Glucose in Bioprocess Media. Int. J. Mol. Sci. 2017, 18, 1235.

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