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Int. J. Mol. Sci. 2017, 18(6), 1233; doi:10.3390/ijms18061233

Interactive Roles of DNA Helicases and Translocases with the Single-Stranded DNA Binding Protein RPA in Nucleic Acid Metabolism

Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health,NIH Biomedical Research Center, 251 Bayview Blvd, Baltimore, MD 21224, USA
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Author to whom correspondence should be addressed.
Academic Editor: Vassilis G. Gorgoulis
Received: 25 April 2017 / Revised: 30 May 2017 / Accepted: 1 June 2017 / Published: 8 June 2017
(This article belongs to the Special Issue Mechanisms Leading to Genomic Instability)
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Abstract

Helicases and translocases use the energy of nucleoside triphosphate binding and hydrolysis to unwind/resolve structured nucleic acids or move along a single-stranded or double-stranded polynucleotide chain, respectively. These molecular motors facilitate a variety of transactions including replication, DNA repair, recombination, and transcription. A key partner of eukaryotic DNA helicases/translocases is the single-stranded DNA binding protein Replication Protein A (RPA). Biochemical, genetic, and cell biological assays have demonstrated that RPA interacts with these human molecular motors physically and functionally, and their association is enriched in cells undergoing replication stress. The roles of DNA helicases/translocases are orchestrated with RPA in pathways of nucleic acid metabolism. RPA stimulates helicase-catalyzed DNA unwinding, enlists translocases to sites of action, and modulates their activities in DNA repair, fork remodeling, checkpoint activation, and telomere maintenance. The dynamic interplay between DNA helicases/translocases and RPA is just beginning to be understood at the molecular and cellular levels, and there is still much to be learned, which may inform potential therapeutic strategies. View Full-Text
Keywords: helicase; translocase; Replication Protein A; RPA; DNA repair; replication; telomere; checkpoint helicase; translocase; Replication Protein A; RPA; DNA repair; replication; telomere; checkpoint
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Awate, S.; Brosh, Jr., R.M. Interactive Roles of DNA Helicases and Translocases with the Single-Stranded DNA Binding Protein RPA in Nucleic Acid Metabolism. Int. J. Mol. Sci. 2017, 18, 1233.

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