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Int. J. Mol. Sci. 2017, 18(6), 1127; doi:10.3390/ijms18061127

Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish

1
Department of Medical Cell Biology and Genetics, College of Preclinical Medicine, Southwest Medical University, Luzhou 646000, China
2
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
3
University of Chinese Academy of Sciences, Beijing 100049, China
*
Author to whom correspondence should be addressed.
Academic Editor: Céline Audet
Received: 14 February 2017 / Revised: 20 April 2017 / Accepted: 18 May 2017 / Published: 7 June 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [12906 KB, uploaded 7 June 2017]   |  

Abstract

The regularity of Piwi-interacting RNA (piRNA) biogenesis is crucial to germline development. Functioning as Piwi-interacting proteins, Tudor domain-related proteins (Tdrds) have been demonstrated to be involved in spermatogenesis and the piRNA pathway. In this study, zebrafish tdrd12 was identified, and the maternal and germ cell-specific expression patterns of zebrafish tdrd12 were observed. Utilizing TALEN (transcription activator-like effector nuclease) techniques, two independent tdrd12 mutant zebrafish lines were generated. Although no defects were found during the generation of the primordial germ cells (PGCs) in the tdrd12-null fish progenies obtained from the heterozygous tdrd12 mutant parents, all Tdrd12-deficient fish developed into infertile males. The reduced numbers and eventually loss of the germ cells by 35 days post fertilization (dpf) led to masculinization and infertility of the Tdrd12-deficient fish. Meiosis defects of the germ cells in the tdrd12 mutants during the gonad-transitioning period were observed, revealing the indispensable functions of Tdrd12 in gametogenesis. Our studies demonstrated that zebrafish Tdrd12 is essential for germ cell development and maintenance. View Full-Text
Keywords: tdrd12; knockout; germ cell; differentiation; maintenance; zebrafish tdrd12; knockout; germ cell; differentiation; maintenance; zebrafish
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Dai, X.; Shu, Y.; Lou, Q.; Tian, Q.; Zhai, G.; Song, J.; Lu, S.; Yu, H.; He, J.; Yin, Z. Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish. Int. J. Mol. Sci. 2017, 18, 1127.

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