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Int. J. Mol. Sci. 2017, 18(5), 968; doi:10.3390/ijms18050968

Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production

Department of Developmental Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, 77 Puhe Road, Shenbei New District, Shenyang 110122, China
These authors contributed equally to this study.
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Authors to whom correspondence should be addressed.
Academic Editor: Shaker A. Mousa
Received: 24 March 2017 / Revised: 25 April 2017 / Accepted: 28 April 2017 / Published: 3 May 2017
(This article belongs to the Special Issue Vascular Biology and Therapeutics)
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Abstract

The formation of brain vasculature is an essential step during central nervous system development. The molecular mechanism underlying brain angiogenesis remains incompletely understood. The role of Atg7, an autophagy-related protein, in brain angiogenesis was investigated in this study. We found that the microvessel density in mice brains with endothelial-specific knockout of Atg7 (Atg7 EKO) was significantly decreased compared to wild-type control. Consistently, in vitro angiogenesis assays showed that Atg7 knockdown impaired angiogenesis in brain microvascular endothelial cells. Further results indicated that knockdown of Atg7 reduced interleukin-6 (IL-6) expression in brain microvascular endothelial cells, which is mediated by NF-κB-dependent transcriptional control. Interestingly, exogenous IL-6 restored the impaired angiogenesis and reduced cell motility caused by Atg7 knockdown. These results demonstrated that Atg7 has proangiogenic activity in brain angiogenesis which is mediated by IL-6 production in a NF-κB-dependent manner. View Full-Text
Keywords: Atg7; angiogenesis; brain microvascular endothelial cell; IL-6; NF-κB Atg7; angiogenesis; brain microvascular endothelial cell; IL-6; NF-κB
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MDPI and ACS Style

Zhuang, S.-F.; Liu, D.-X.; Wang, H.-J.; Zhang, S.-H.; Wei, J.-Y.; Fang, W.-G.; Zhang, K.; Cao, L.; Zhao, W.-D.; Chen, Y.-H. Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production. Int. J. Mol. Sci. 2017, 18, 968.

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