Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production
AbstractThe formation of brain vasculature is an essential step during central nervous system development. The molecular mechanism underlying brain angiogenesis remains incompletely understood. The role of Atg7, an autophagy-related protein, in brain angiogenesis was investigated in this study. We found that the microvessel density in mice brains with endothelial-specific knockout of Atg7 (Atg7 EKO) was significantly decreased compared to wild-type control. Consistently, in vitro angiogenesis assays showed that Atg7 knockdown impaired angiogenesis in brain microvascular endothelial cells. Further results indicated that knockdown of Atg7 reduced interleukin-6 (IL-6) expression in brain microvascular endothelial cells, which is mediated by NF-κB-dependent transcriptional control. Interestingly, exogenous IL-6 restored the impaired angiogenesis and reduced cell motility caused by Atg7 knockdown. These results demonstrated that Atg7 has proangiogenic activity in brain angiogenesis which is mediated by IL-6 production in a NF-κB-dependent manner. View Full-Text
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Zhuang, S.-F.; Liu, D.-X.; Wang, H.-J.; Zhang, S.-H.; Wei, J.-Y.; Fang, W.-G.; Zhang, K.; Cao, L.; Zhao, W.-D.; Chen, Y.-H. Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production. Int. J. Mol. Sci. 2017, 18, 968.
Zhuang S-F, Liu D-X, Wang H-J, Zhang S-H, Wei J-Y, Fang W-G, Zhang K, Cao L, Zhao W-D, Chen Y-H. Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production. International Journal of Molecular Sciences. 2017; 18(5):968.Chicago/Turabian Style
Zhuang, Shi-Fang; Liu, Dong-Xin; Wang, Hui-Jie; Zhang, Shu-Hong; Wei, Jia-Yi; Fang, Wen-Gang; Zhang, Ke; Cao, Liu; Zhao, Wei-Dong; Chen, Yu-Hua. 2017. "Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production." Int. J. Mol. Sci. 18, no. 5: 968.
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