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Int. J. Mol. Sci. 2017, 18(1), 156; doi:10.3390/ijms18010156

Ftr82 Is Critical for Vascular Patterning during Zebrafish Development

1
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan
2
Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan
3
Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
4
Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan
5
Department of Bioagricultural Science, National Chiayi University, Chiayi City 60004, Taiwan
6
Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
7
Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung 80424, Taiwan
8
Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 80424, Taiwan
9
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan
The authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Shaker A. Mousa
Received: 19 August 2016 / Revised: 4 January 2017 / Accepted: 5 January 2017 / Published: 13 January 2017
(This article belongs to the Special Issue Vascular Biology and Therapeutics)
View Full-Text   |   Download PDF [13815 KB, uploaded 13 January 2017]   |  

Abstract

Cellular components and signaling pathways are required for the proper growth of blood vessels. Here, we report for the first time that a teleost-specific gene ftr82 (finTRIM family, member 82) plays a critical role in vasculature during zebrafish development. To date, there has been no description of tripartite motif proteins (TRIM) in vascular development, and the role of ftr82 is unknown. In this study, we found that ftr82 mRNA is expressed during the development of vessels, and loss of ftr82 by morpholino (MO) knockdown impairs the growth of intersegmental vessels (ISV) and caudal vein plexus (CVP), suggesting that ftr82 plays a critical role in promoting ISV and CVP growth. We showed the specificity of ftr82 MO by analyzing ftr82 expression products and expressing ftr82 mRNA to rescue ftr82 morphants. We further showed that the knockdown of ftr82 reduced ISV cell numbers, suggesting that the growth impairment of vessels is likely due to a decrease of cell proliferation and migration, but not cell death. In addition, loss of ftr82 affects the expression of vascular markers, which is consistent with the defect of vascular growth. Finally, we showed that ftr82 likely interacts with vascular endothelial growth factor (VEGF) and Notch signaling. Together, we identify teleost-specific ftr82 as a vascular gene that plays an important role for vascular development in zebrafish. View Full-Text
Keywords: ftr82; TRIM family; angiogenesis; ISV (intersegmental vessel); CVP (caudal vein plexus); zebrafish ftr82; TRIM family; angiogenesis; ISV (intersegmental vessel); CVP (caudal vein plexus); zebrafish
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MDPI and ACS Style

Chang, H.-W.; Wang, W.-D.; Chiu, C.-C.; Chen, C.-H.; Wang, Y.-S.; Chen, Z.-Y.; Liu, W.; Tai, M.-H.; Wen, Z.-H.; Wu, C.-Y. Ftr82 Is Critical for Vascular Patterning during Zebrafish Development. Int. J. Mol. Sci. 2017, 18, 156.

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