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Int. J. Mol. Sci. 2017, 18(5), 1027; doi:10.3390/ijms18051027

The Effect of VPA on Increasing Radiosensitivity in Osteosarcoma Cells and Primary-Culture Cells from Chemical Carcinogen-Induced Breast Cancer in Rats

1
Department of Occupational Health and Occupational Medicine, School of Public Health, Shandong University, Jinan 250012, China
2
Flinders Rural Health South Australia, Victor Harbor, SA 5211, Australia
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: Ashis Basu and Takehiko Nohmi
Received: 30 March 2017 / Revised: 30 April 2017 / Accepted: 5 May 2017 / Published: 10 May 2017
(This article belongs to the Special Issue Chemically-Induced DNA Damage, Mutagenesis, and Cancer)
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Abstract

This study explored whether valproic acid (VPA, a histone deacetylase inhibitor) could radiosensitize osteosarcoma and primary-culture tumor cells, and determined the mechanism of VPA-induced radiosensitization. The working system included osteosarcoma cells (U2OS) and primary-culture cells from chemical carcinogen (DMBA)-induced breast cancer in rats; and clonogenic survival, immunofluorescence, fluorescent in situ hybridization (FISH) for chromosome aberrations, and comet assays were used in this study. It was found that VPA at the safe or critical safe concentration of 0.5 or 1.0 mM VPA could result in the accumulation of more ionizing radiation (IR)-induced DNA double strand breaks, and increase the cell radiosensitivity. VPA-induced radiosensitivity was associated with the inhibition of DNA repair activity in the working systems. In addition, the chromosome aberrations including chromosome breaks, chromatid breaks, and radial structures significantly increased after the combination treatment of VPA and IR. Importantly, the results obtained by primary-culture cells from the tissue of chemical carcinogen-induced breast cancer in rats further confirmed our findings. The data in this study demonstrated that VPA at a safe dose was a radiosensitizer for osteosarcoma and primary-culture tumor cells through suppressing DNA-double strand breaks repair function. View Full-Text
Keywords: VPA; DNA double-strand breaks; radiosensitivity; DNA repair; U2OS; chemical carcinogen (DMBA)-induced tumor VPA; DNA double-strand breaks; radiosensitivity; DNA repair; U2OS; chemical carcinogen (DMBA)-induced tumor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liu, G.; Wang, H.; Zhang, F.; Tian, Y.; Tian, Z.; Cai, Z.; Lim, D.; Feng, Z. The Effect of VPA on Increasing Radiosensitivity in Osteosarcoma Cells and Primary-Culture Cells from Chemical Carcinogen-Induced Breast Cancer in Rats. Int. J. Mol. Sci. 2017, 18, 1027.

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