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Int. J. Mol. Sci. 2017, 18(5), 927; doi:10.3390/ijms18050927

Identification and Expression Profiling of the Auxin Response Factors in Dendrobium officinale under Abiotic Stresses

1
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
2
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 310036, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 14 March 2017 / Revised: 24 April 2017 / Accepted: 25 April 2017 / Published: 4 May 2017
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [12922 KB, uploaded 4 May 2017]   |  

Abstract

Auxin response factor (ARF) proteins play roles in plant responses to diverse environmental stresses by binding specifically to the auxin response element in the promoters of target genes. Using our latest public Dendrobium transcriptomes, a comprehensive characterization and analysis of 14 DnARF genes were performed. Three selected DnARFs, including DnARF1, DnARF4, and DnARF6, were confirmed to be nuclear proteins according to their transient expression in epidermal cells of Nicotiana benthamiana leaves. Furthermore, the transcription activation abilities of DnARF1, DnARF4, and DnARF6 were tested in a yeast system. Our data showed that DnARF6 is a transcriptional activator in Dendrobium officinale. To uncover the basic information of DnARF gene responses to abiotic stresses, we analyzed their expression patterns under various hormones and abiotic treatments. Based on our data, several hormones and significant stress responsive DnARF genes have been identified. Since auxin and ARF genes have been identified in many plant species, our data is imperative to reveal the function of ARF mediated auxin signaling in the adaptation to the challenging Dendrobium environment. View Full-Text
Keywords: abiotic stress; auxin; ARF; Dendrobium officinale; transcriptional activator abiotic stress; auxin; ARF; Dendrobium officinale; transcriptional activator
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Chen, Z.; Yuan, Y.; Fu, D.; Shen, C.; Yang, Y. Identification and Expression Profiling of the Auxin Response Factors in Dendrobium officinale under Abiotic Stresses. Int. J. Mol. Sci. 2017, 18, 927.

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