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Int. J. Mol. Sci. 2017, 18(5), 1046; doi:10.3390/ijms18051046

Identifying the Genes Regulated by AtWRKY6 Using Comparative Transcript and Proteomic Analysis under Phosphorus Deficiency

College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
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Author to whom correspondence should be addressed.
Academic Editors: Yikun He, Xuchu Wang and Shaojun Dai
Received: 8 February 2017 / Revised: 26 April 2017 / Accepted: 4 May 2017 / Published: 12 May 2017
(This article belongs to the Special Issue Selected Papers from the 6th National Plant Protein Research Congress)
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Abstract

Phosphorus (P) is an important mineral nutrient for plant growth and development. Overexpressing AtWRKY6 (35S:WRKY6-9) was more sensitive and wrky6 (wrky6-1) was more resistant under low Pi conditions. To better understand the function of AtWRKY6 under low phosphate stress conditions, we applied two-dimensional gel electrophoresis (2-DE) to analyse differentially expressed proteins in the shoots and roots between wild type, 35S:WRKY6-9 and wrky6-1 after phosphorus deficiency treatment for three days. The results showed 88 differentially abundant protein spots, which were identified between the shoots and roots of 35S:WRKY6-9 and wrky6-1 plants. In addition, 59 differentially expressed proteins were identified in the leaves and roots of 35S:WRKY6-9 plants. After analysis, 9 genes with W-box elements in their promoter sequences were identified in the leaves, while 6 genes with W-box elements in their promoter sequences were identified in the roots. A total of 8 genes were identified as potential target genes according to the quantitative PCR (QPCR) and two dimension difference gel electrophoresis, (2D-DIGE) results, including ATP synthase, gln synthetase, nitrilase, 14-3-3 protein, carbonic anhydrases 2, and tryptophan synthase. These results provide important information concerning the AtWRKY6 regulation network and reveal potential vital target genes of AtWRKY6 under low phosphorus stress. two dimension difference gel electrophoresis, 2D-DIGE View Full-Text
Keywords: phosphorus deficiency; AtWRKY6; proteomics analysis phosphorus deficiency; AtWRKY6; proteomics analysis
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Li, L.-Q.; Huang, L.-P.; Pan, G.; Liu, L.; Wang, X.-Y.; Lu, L.-M. Identifying the Genes Regulated by AtWRKY6 Using Comparative Transcript and Proteomic Analysis under Phosphorus Deficiency. Int. J. Mol. Sci. 2017, 18, 1046.

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