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Int. J. Mol. Sci. 2017, 18(4), 838; doi:10.3390/ijms18040838

Applying Broadband Dielectric Spectroscopy (BDS) for the Biophysical Characterization of Mammalian Tissues under a Variety of Cellular Stresses

1
Physics Department, School of Applied Mathematical and Physical Sciences, National Technical University of Athens, Zografou Campus, 15780 Athens, Greece
2
Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, 15701 Athens, Greece
3
Molecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, University of Athens, 11527 Athens, Greece
4
Laboratory Animal Facilities, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation, Academy of Athens, 4 Soranou Efesiou Street, 11527 Athens, Greece
5
Diagnostic Echotomography Medical S.A., 317C Kifissias Avenue, 145 61 Kifissia, Greece
6
Department of Pathology, Onassis Cardiac Surgery Center, 356 Sygrou Avenue, 17674 Kallithea, Greece
7
Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications, National Centre for Scientific Research “Demokritos”, 60037 Athens, Greece
Present address: Department of Neuroscience, Karolinska Institutet, 17177 Stockholm, Sweden
*
Author to whom correspondence should be addressed.
Received: 28 February 2017 / Revised: 10 April 2017 / Accepted: 12 April 2017 / Published: 15 April 2017
(This article belongs to the Special Issue Mechanisms Leading to Genomic Instability)
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Abstract

The dielectric properties of biological tissues can contribute non-invasively to a better characterization and understanding of the structural properties and physiology of living organisms. The question we asked, is whether these induced changes are effected by an endogenous or exogenous cellular stress, and can they be detected non-invasively in the form of a dielectric response, e.g., an AC conductivity switch in the broadband frequency spectrum. This study constitutes the first methodological approach for the detection of environmental stress-induced damage in mammalian tissues by the means of broadband dielectric spectroscopy (BDS) at the frequencies of 1–106 Hz. Firstly, we used non-ionizing (NIR) and ionizing radiation (IR) as a typical environmental stress. Specifically, rats were exposed to either digital enhanced cordless telecommunication (DECT) radio frequency electromagnetic radiation or to γ-radiation, respectively. The other type of stress, characterized usually by high genomic instability, was the pathophysiological state of human cancer (lung and prostate). Analyzing the results of isothermal dielectric measurements provided information on the tissues’ water fraction. In most cases, our methodology proved sufficient in detecting structural changes, especially in the case of IR and malignancy. Useful specific dielectric response patterns are detected and correlated with each type of stress. Our results point towards the development of a dielectric-based methodology for better understanding and, in a relatively invasive way, the biological and structural changes effected by radiation and developing lung or prostate cancer often associated with genomic instability. View Full-Text
Keywords: broadband dielectric spectroscopy; radiation; tissues; cancer; cellular stress; genomic instability broadband dielectric spectroscopy; radiation; tissues; cancer; cellular stress; genomic instability
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Souli, M.P.; Klonos, P.; Fragopoulou, A.F.; Mavragani, I.V.; Pateras, I.S.; Kostomitsopoulos, N.; Margaritis, L.H.; Zoumpoulis, P.; Kaklamanis, L.; Kletsas, D.; Gorgoulis, V.G.; Kyritsis, A.; Pissis, P.; Georgakilas, A.G. Applying Broadband Dielectric Spectroscopy (BDS) for the Biophysical Characterization of Mammalian Tissues under a Variety of Cellular Stresses. Int. J. Mol. Sci. 2017, 18, 838.

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