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Int. J. Mol. Sci. 2017, 18(1), 87; doi:10.3390/ijms18010087

Evaluation of Magnetic Nanoparticle-Labeled Chondrocytes Cultivated on a Type II Collagen–Chitosan/Poly(Lactic-co-Glycolic) Acid Biphasic Scaffold

1
Department of Surgery, Chang Gung Memorial Hospital, Keelung 204, Taiwan
2
Department of Orthopaedic Surgery, Wan Fang Hospital, School of Medicine, College of Medicine, Taipei Medial University, Taipei 116, Taiwan
3
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Cory J. Xian
Received: 9 September 2016 / Revised: 26 December 2016 / Accepted: 29 December 2016 / Published: 4 January 2017
(This article belongs to the Special Issue Advances in Bone and Cartilage Research)
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Abstract

Chondral or osteochondral defects are still controversial problems in orthopedics. Here, chondrocytes labeled with magnetic nanoparticles were cultivated on a biphasic, type II collagen–chitosan/poly(lactic-co-glycolic acid) scaffold in an attempt to develop cultures with trackable cells exhibiting growth, differentiation, and regeneration. Rabbit chondrocytes were labeled with magnetic nanoparticles and characterized by scanning electron microscopy (SEM), transmission electron (TEM) microscopy, and gene and protein expression analyses. The experimental results showed that the magnetic nanoparticles did not affect the phenotype of chondrocytes after cell labeling, nor were protein and gene expression affected. The biphasic type II collagen–chitosan/poly(lactic-co-glycolic) acid scaffold was characterized by SEM, and labeled chondrocytes showed a homogeneous distribution throughout the scaffold after cultivation onto the polymer. Cellular phenotype remained unaltered but with increased gene expression of type II collagen and aggrecan, as indicated by cell staining, indicating chondrogenesis. Decreased SRY-related high mobility group-box gene (Sox-9) levels of cultured chondrocytes indicated that differentiation was associated with osteogenesis. These results are encouraging for the development of techniques for trackable cartilage regeneration and osteochondral defect repair which may be applied in vivo and, eventually, in clinical trials. View Full-Text
Keywords: chondrocytes; type II collagen; chitosan; poly(lactic-co-glycolic acid); magnetic nanoparticles; biphasic scaffold chondrocytes; type II collagen; chitosan; poly(lactic-co-glycolic acid); magnetic nanoparticles; biphasic scaffold
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MDPI and ACS Style

Su, J.-Y.; Chen, S.-H.; Chen, Y.-P.; Chen, W.-C. Evaluation of Magnetic Nanoparticle-Labeled Chondrocytes Cultivated on a Type II Collagen–Chitosan/Poly(Lactic-co-Glycolic) Acid Biphasic Scaffold. Int. J. Mol. Sci. 2017, 18, 87.

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