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Open AccessCommunication
Int. J. Mol. Sci. 2017, 18(1), 45; doi:10.3390/ijms18010045

Simultaneous Determination and Pharmacokinetic Study of Six Components in Rat Plasma by HPLC-MS/MS after Oral Administration of Acanthopanax sessiliflorus Fruit Extract

1
College of Pharmacy, Soochow University, Suzhou 215123, China
2
Drug Clinical Trial Institution, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China
3
Department of Pharmacy, Jiangxi Maternal and Child Health Hospital, No. 318 Bayi Road, Nanchang 330001, China
4
College of Pharmacy, Liaoning University, Shenyang 110036, China
*
Author to whom correspondence should be addressed.
Academic Editor: David Arráez-Román
Received: 13 September 2016 / Revised: 8 December 2016 / Accepted: 12 December 2016 / Published: 28 December 2016
(This article belongs to the Section Bioactives and Nutraceuticals)
View Full-Text   |   Download PDF [1487 KB, uploaded 28 December 2016]   |  

Abstract

A specific and reliable HPLC-MS/MS method was developed and validated for the simultaneous determination of protocatechuic acid (PCA), scopolin, (−)-pinoresinol-4,4′-di-O-β-d-glucopyranoside (PDG), acanthoside D, acanthoside B and hyperin in rat plasma for the first time. The analytes were separated on a C18 column (50 × 2.1 mm, 1.8 µm) and a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source was used for detection. The rat plasma sample was prepared using the protein precipitation procedure. The calibration curves were linear over a concentration range of 1.2–1200.0 ng/mL for PCA, 0.96–960.0 ng/mL for scopolin, 1.12–1120.0 ng/mL for PDG, 1.32–1320.0 ng/mL for acanthoside D, 0.99–990.0 ng/mL for acanthoside B and 1.01–1010.0 ng/mL for hyperin. The intra-day and inter-day precision was less than 11.4% and the relative error (RE) was all within ±15%. The validated method was successfully applied to assess the pharmacokinetics characteristics after the extracts of Acanthopanax sessiliflorus fruits were orally administered to the Sprague-Dawley rat. View Full-Text
Keywords: HPLC-MS/MS; Acanthopanax sessiliflorus fruits; rat plasma; pharmacokinetics HPLC-MS/MS; Acanthopanax sessiliflorus fruits; rat plasma; pharmacokinetics
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Du, P.; Lei, M.; Liu, Y.; Yang, S. Simultaneous Determination and Pharmacokinetic Study of Six Components in Rat Plasma by HPLC-MS/MS after Oral Administration of Acanthopanax sessiliflorus Fruit Extract. Int. J. Mol. Sci. 2017, 18, 45.

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