Pnserpin: A Novel Serine Protease Inhibitor from Extremophile Pyrobaculum neutrophilum
AbstractSerine protease inhibitors (serpins) are native inhibitors of serine proteases, constituting a large protein family with members spread over eukaryotes and prokaryotes. However, only very few prokaryotic serpins, especially from extremophiles, have been characterized to date. In this study, Pnserpin, a putative serine protease inhibitor from the thermophile Pyrobaculum neutrophilum, was overexpressed in Escherichia coli for purification and characterization. It irreversibly inhibits chymotrypsin-, trypsin-, elastase-, and subtilisin-like proteases in a temperature range from 20 to 100 °C in a concentration-dependent manner. The stoichiometry of inhibition (SI) of Pnserpin for proteases decreases as the temperature increases, indicating that the inhibitory activity of Pnserpin increases with the temperature. SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) showed that Pnserpin inhibits proteases by forming a SDS-resistant covalent complex. Homology modeling and molecular dynamic simulations predicted that Pnserpin can form a stable common serpin fold. Results of the present work will help in understanding the structural and functional characteristics of thermophilic serpin and will broaden the current knowledge about serpins from extremophiles. View Full-Text
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Zhang, H.; Fei, R.; Xue, B.; Yu, S.; Zhang, Z.; Zhong, S.; Gao, Y.; Zhou, X. Pnserpin: A Novel Serine Protease Inhibitor from Extremophile Pyrobaculum neutrophilum. Int. J. Mol. Sci. 2017, 18, 113.
Zhang H, Fei R, Xue B, Yu S, Zhang Z, Zhong S, Gao Y, Zhou X. Pnserpin: A Novel Serine Protease Inhibitor from Extremophile Pyrobaculum neutrophilum. International Journal of Molecular Sciences. 2017; 18(1):113.Chicago/Turabian Style
Zhang, Huan; Fei, Rui; Xue, Baigong; Yu, Shanshan; Zhang, Zuoming; Zhong, Sheng; Gao, Yuanqi; Zhou, Xiaoli. 2017. "Pnserpin: A Novel Serine Protease Inhibitor from Extremophile Pyrobaculum neutrophilum." Int. J. Mol. Sci. 18, no. 1: 113.
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