Next Article in Journal
The Impact of Growth Hormone Therapy on the Apoptosis Assessment in CD34+ Hematopoietic Cells from Children with Growth Hormone Deficiency
Next Article in Special Issue
From Clinical Standards to Translating Next-Generation Sequencing Research into Patient Care Improvement for Hepatobiliary and Pancreatic Cancers
Previous Article in Journal
Pharmacogenetic Foundations of Therapeutic Efficacy and Adverse Events of Statins
Previous Article in Special Issue
A Novel Pathogenic BRCA1 Splicing Variant Produces Partial Intron Retention in the Mature Messenger RNA
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(1), 109; doi:10.3390/ijms18010109

The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing

1
CEINGE-Biotecnologie Avanzate, via G. Salvatore 486, 80145 Naples, Italy
2
Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via Pansini 5, 80131 Naples, Italy
3
Laboratory of Archeo-Anthropology, University of Naples Suor Orsola Benincasa, via Suor Orsola 10, 80125 Naples, Italy
4
Department of Public Health, University of Naples Federico II, Naples, via Pansini 5, 80131 Naples, Italy
5
Microbiology Unit, Hospital of Cosenza, via San Martino, 87100 Cosenza, Italy
6
Department of Advanced Biomedical Sciences, University of Naples Federico II, via Pansini 5, 80131 Naples, Italy
7
IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico)-Fondazione SDN, via Gianturco 113, 80143 Naples, Italy
Present address: Life Sciences Lab, University of Florida, Kennedy Space Center, Merritt Island, FL 32953, USA.
*
Authors to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 28 October 2016 / Revised: 29 December 2016 / Accepted: 3 January 2017 / Published: 6 January 2017
(This article belongs to the Special Issue Next-Generation Sequencing for Clinical Application)
View Full-Text   |   Download PDF [3317 KB, uploaded 11 January 2017]   |  

Abstract

The history of medicine abounds in cases of mysterious deaths, especially by infectious diseases, which were probably unresolved because of the lack of knowledge and of appropriate technology. The aim of this study was to exploit contemporary technologies to try to identify the cause of death of a young boy who died from a putative “infection” at the end of the 18th century, and for whom an extraordinarily well-preserved minute bone fragment was available. After confirming the nature of the sample, we used laser microdissection to select the most “informative” area to be examined. Tissue genotyping indicated male gender, thereby confirming the notary’s report. 16S ribosomal RNA sequencing showed that Proteobacteria and Actinobacteria were more abundant than Firmicutes and Bacteroidetes, and that Pseudomonas was the most abundant bacterial genus in the Pseudomonadaceae family. These data suggest that the patient most likely died from Pseudomonas osteomyelitis. This case is an example of how new technological approaches, like laser microdissection and next-generation sequencing, can resolve ancient cases of uncertain etiopathology. Lastly, medical samples may contain a wealth of information that may not be accessible until more sophisticated technology becomes available. Therefore, one may envisage the possibility of systematically storing medical samples for evaluation by future generations. View Full-Text
Keywords: metagenomics; human microbiome; cold case; next generation sequencing metagenomics; human microbiome; cold case; next generation sequencing
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

D’Argenio, V.; Torino, M.; Precone, V.; Casaburi, G.; Esposito, M.V.; Iaffaldano, L.; Malapelle, U.; Troncone, G.; Coto, I.; Cavalcanti, P.; De Rosa, G.; Salvatore, F.; Sacchetti, L. The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing. Int. J. Mol. Sci. 2017, 18, 109.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top