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Int. J. Mol. Sci. 2017, 18(1), 111; doi:10.3390/ijms18010111

The Impact of Growth Hormone Therapy on the Apoptosis Assessment in CD34+ Hematopoietic Cells from Children with Growth Hormone Deficiency

1
Department of General Pathology, Pomeranian Medical University in Szczecin, 72 Powstancow Wlkp. Street, 70-111 Szczecin, Poland
2
Department of Pediatrics, Endocrinology, Diabetology, Metabolic Diseases and Cardiology of the Developmental Age, Pomeranian Medical University in Szczecin, 1 Unii Lubelskiej Street, 71-252 Szczecin, Poland
3
Department of Pediatric and Oncological Surgery, Pomeranian Medical University in Szczecin, 1 Unii Lubelskiej Street, 71-252 Szczecin, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Anthony Lemarié
Received: 18 August 2016 / Revised: 10 December 2016 / Accepted: 21 December 2016 / Published: 7 January 2017
(This article belongs to the Collection Programmed Cell Death and Apoptosis)
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Abstract

Growth hormone (GH) modulates hematopoietic cell homeostasis and is associated with apoptosis control, but with limited mechanistic insights. Aim of the study was to determine whether GH therapeutic supplementation (GH-TS) could affect apoptosis of CD34+ cells enriched in hematopoietic progenitor cells of GH deficient (GHD) children. CD34+ cells from peripheral blood of 40 GHD children were collected before and in 3rd and 6th month of GH-TS and compared to 60 controls adjusted for bone age, sex, and pubertal development. Next, apoptosis assessment via different molecular techniques was performed. Finally, to comprehensively characterize apoptosis process, global gene expression profile was determined using genome-wide RNA microarray technology. Results showed that GH-TS significantly reduced spontaneous apoptosis in CD34+ cells (p < 0.01) and results obtained using different methods to detect early and late apoptosis in analyzed cells population were consistent. GH-TS was also associated with significant downregulation of several members of TNF-alpha superfamily and other genes associated with apoptosis and stress response. Moreover, the significant overexpression of cyto-protective and cell cycle-associated genes was detected. These findings suggest that recombinant human GH has a direct anti-apoptotic activity in hematopoietic CD34+ cells derived from GHD subjects in course of GH-TS. View Full-Text
Keywords: CD34+ cells; hematopoietic progenitor cells; hematopoiesis; growth hormone deficiency; growth hormone deficiency in children; apoptosis; cell death; apoptosis control CD34+ cells; hematopoietic progenitor cells; hematopoiesis; growth hormone deficiency; growth hormone deficiency in children; apoptosis; cell death; apoptosis control
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Kawa, M.P.; Stecewicz, I.; Piecyk, K.; Paczkowska, E.; Rogińska, D.; Sobuś, A.; Łuczkowska, K.; Pius-Sadowska, E.; Gawrych, E.; Petriczko, E.; Walczak, M.; Machaliński, B. The Impact of Growth Hormone Therapy on the Apoptosis Assessment in CD34+ Hematopoietic Cells from Children with Growth Hormone Deficiency. Int. J. Mol. Sci. 2017, 18, 111.

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