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Int. J. Mol. Sci. 2016, 17(9), 1477; doi:10.3390/ijms17091477

Nicotine Suppressed Fetal Adrenal StAR Expression via YY1 Mediated-Histone Deacetylation Modification Mechanism

1
Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071, China
2
Department of Pharmacology, Medical School of Yangtze University, Jingzhou 434000, China
*
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 19 June 2016 / Revised: 10 August 2016 / Accepted: 29 August 2016 / Published: 3 September 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Steroidogenic acute regulatory (StAR) protein plays a pivotal role in steroidogenesis. Previously, we have demonstrated that prenatal nicotine exposure suppressed fetal adrenal steroidogenesis via steroidogenic factor 1 deacetylation. This study further explored the potential role of the transcriptional repressor Yin Yang 1 (YY1) in nicotine-mediated StAR inhibition. Nicotine was subcutaneously administered (1.0 mg/kg) to pregnant rats twice per day and NCI-H295A cells were treated with nicotine. StAR and YY1 expression were analyzed by real-time PCR, immunohistochemistry, and Western blotting. Histone modifications and the interactions between the YY1 and StAR promoter were assessed using chromatin immunoprecipitation (ChIP). Prenatal nicotine exposure increased YY1 expression and suppressed StAR expression. ChIP assay showed that there was a decreasing trend for histone acetylation at the StAR promoter in fetal adrenal glands, whereas H3 acetyl-K14 at the YY1 promoter presented an increasing trend following nicotine exposure. Furthermore, in nicotine-treated NCI-H295A cells, nicotine enhanced YY1 expression and inhibited StAR expression. ChIP assay showed that histone acetylation decreased at the StAR promoter in NCI-H295A cells and that the interaction between the YY1 and StAR promoter increased. These data indicated that YY1-medicated histone deacetylation modification in StAR promoters might play an important role in the inhibitory effect of nicotine on StAR expression. View Full-Text
Keywords: nicotine; Yin Yang 1 (YY1); steroidogenic acute regulatory (StAR); fetal adrenal; histone deacetylation nicotine; Yin Yang 1 (YY1); steroidogenic acute regulatory (StAR); fetal adrenal; histone deacetylation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, L.; Wang, J.-F.; Fan, J.; Rao, Y.-S.; Liu, F.; Yan, Y.-E.; Wang, H. Nicotine Suppressed Fetal Adrenal StAR Expression via YY1 Mediated-Histone Deacetylation Modification Mechanism. Int. J. Mol. Sci. 2016, 17, 1477.

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