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Int. J. Mol. Sci. 2016, 17(9), 1404; doi:10.3390/ijms17091404

Wnt/β-Catenin Signaling Mediated-UCH-L1 Expression in Podocytes of Diabetic Nephropathy

1
Department of Clinic Pathology, Weifang Medical University, Weifang 261053, China
2
Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
3
Department of Pathology, Affiliated Hospital of Weifang Medical University, Weifang 261031, China
4
Department of Pathology, Maternal and Child Care Service Centre of Changyi, Changyi 261300, China
*
Author to whom correspondence should be addressed.
Academic Editors: Yuehui Wang and Zhiguo Zhang
Received: 8 July 2016 / Revised: 7 August 2016 / Accepted: 18 August 2016 / Published: 25 August 2016
(This article belongs to the Special Issue Diabetic Complications: Pathophysiology, Mechanisms, and Therapies)
View Full-Text   |   Download PDF [8355 KB, uploaded 25 August 2016]   |  

Abstract

Increasing studies identified podocyte injury as a key early risk factor resulting in diabetic nephropathy (DN). The ubiquitin carboxy-terminal hydrolase 1 (UCH-L1) participates in podocyte differentiation and injury, which is elevated in the podocytes of a variety of nephritis. Whether UCH-L1 expression is positively related to podocyte injury of DN remains unclear. In this study, elevated expression of UCH-L1 and its intrinsic mechanism in high glucose (HG)-stimulated murine podocytes were investigated using western blot and real-time quantitative PCR. Kidney biopsies of DN patients and health individuals were stained by immunofluorescence (IF) method. The morphological and functional changes of podocytes were tested by F-actin staining and cell migration assay. Results demonstrated that HG induced upregulation of UCH-L1 and activation of the Wnt/β-catenin signaling pathway in podocytes. However, blocking of the Wnt pathway by dickkopf related protein 1 (DKK1) eliminated the above changes. Furthermore, IF staining confirmed that, compared with healthy individuals, the expression of UCH-L1 and β-catenin were obviously increased in kidney biopsy of DN patients. Overexpression of UCH-L1 remodeled its actin cytoskeleton, increased its cell migration and impacted its important proteins. All the findings manifested that Wnt/β-catenin/UCH-L1 may be a new potential therapy method in the treatment of DN in future. View Full-Text
Keywords: podocytes; UCH-L1; Wnt/β-catenin; DN podocytes; UCH-L1; Wnt/β-catenin; DN
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MDPI and ACS Style

Zhang, H.; Luo, W.; Sun, Y.; Qiao, Y.; Zhang, L.; Zhao, Z.; Lv, S. Wnt/β-Catenin Signaling Mediated-UCH-L1 Expression in Podocytes of Diabetic Nephropathy. Int. J. Mol. Sci. 2016, 17, 1404.

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