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Int. J. Mol. Sci. 2016, 17(6), 963; doi:10.3390/ijms17060963

Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells

1
Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea
2
Department of Orthopedic Surgery, Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea
3
Department of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 619-961, Korea
4
Department of Biomedical Engineering, Collage of Medicine, Kyung Hee University, Seoul 151-742, Korea
5
Department of Neurosurgery, Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea
6
Department of Food Science and Biotechnology, College of Life Science, CHA University, Gyeonggi-do 443-742, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Masatoshi Maki
Received: 23 April 2016 / Revised: 7 June 2016 / Accepted: 9 June 2016 / Published: 17 June 2016
(This article belongs to the Special Issue Metalloproteins)
View Full-Text   |   Download PDF [5627 KB, uploaded 17 June 2016]   |  

Abstract

Human adipose-derived stem cells (hASCs) have a capacity to undergo adipogenic, chondrogenic, and osteogenic differentiation. Recently, hASCs were applied to various fields including cell therapy for tissue regeneration. However, it is hard to predict the direction of differentiation of hASCs in real-time. Matrix metalloproteinases (MMPs) are one family of proteolytic enzymes that plays a pivotal role in regulating the biology of stem cells. MMPs secreted by hASCs are expected to show different expression patterns depending on the differentiation state of hASCs because biological functions exhibit different patterns during the differentiation of stem cells. Here, we investigated proteolytic enzyme activity, especially MMP-2 activity, in hASCs during their differentiation. The activities of proteolytic enzymes and MMP-2 were higher during chondrogenic differentiation than during adipogenic and osteogenic differentiation. During chondrogenic differentiation, mRNA expression of MMP-2 and the level of the active form of MMP-2 were increased, which also correlated with Col II. It is concluded that proteolytic enzyme activity and the level of the active form of MMP-2 were increased during chondrogenic differentiation, which was accelerated in the presence of Col II protein. According to our findings, MMP-2 could be a candidate maker for real-time detection of chondrogenic differentiation of hASCs. View Full-Text
Keywords: human adipose-derived stem cells; chondrogenic differentiation; extracellular matrix; matrix metalloproteinase-2 human adipose-derived stem cells; chondrogenic differentiation; extracellular matrix; matrix metalloproteinase-2
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Arai, Y.; Park, S.; Choi, B.; Ko, K.-W.; Choi, W.C.; Lee, J.-M.; Han, D.-W.; Park, H.-K.; Han, I.; Lee, J.H.; Lee, S.-H. Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells. Int. J. Mol. Sci. 2016, 17, 963.

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