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Int. J. Mol. Sci. 2016, 17(5), 726; doi:10.3390/ijms17050726

Hybrid Cells Derived from Human Breast Cancer Cells and Human Breast Epithelial Cells Exhibit Differential TLR4 and TLR9 Signaling

1
Institute of Immunology & Experimental Oncology, Center for Biomedical Education and Research (ZBAF), University of Witten/Herdecke, Stockumer Str. 10, 58448 Witten, Germany
2
Faculty of Medicine, Ruhr University Bochum, Universitätsstraße 150, 44801 Bochum, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Terrence Piva
Received: 24 March 2016 / Revised: 15 April 2016 / Accepted: 3 May 2016 / Published: 13 May 2016
(This article belongs to the Special Issue Cell Fusion in Cancer)
View Full-Text   |   Download PDF [4294 KB, uploaded 13 May 2016]   |  

Abstract

TLRs are important receptors of cells of the innate immune system since they recognize various structurally conserved molecular patterns of different pathogens as well as endogenous ligands. In cancer, the role of TLRs is still controversial due to findings that both regression and progression of tumors could depend on TLR signaling. In the present study, M13SV1-EGFP-Neo human breast epithelial cells, MDA-MB-435-Hyg human breast cancer cells and two hybrids M13MDA435-1 and -3 were investigated for TLR4 and TLR9 expression and signaling. RT-PCR data revealed that LPS and CpG-ODN induced the expression of pro-inflammatory cytokines, like IFN-β, TNF-α, IL-1β and IL-6 in hybrid cells, but not parental cells. Interestingly, validation of RT-PCR data by Western blot showed detectable protein levels solely after LPS stimulation, suggesting that regulatory mechanisms are also controlled by TLR signaling. Analysis of pAKT and pERK1/2 levels upon LPS and CpG-ODN stimulation revealed a differential phosphorylation pattern in all cells. Finally, the migratory behavior of the cells was investigated showing that both LPS and CpG-ODN potently blocked the locomotory activity of the hybrid cells in a dose-dependent manner. In summary, hybrid cells exhibit differential TLR4 and TLR9 signaling. View Full-Text
Keywords: cell fusion; breast cancer; TLR4; TLR9; signal transduction cell fusion; breast cancer; TLR4; TLR9; signal transduction
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Tosun, S.; Fried, S.; Niggemann, B.; Zänker, K.S.; Dittmar, T. Hybrid Cells Derived from Human Breast Cancer Cells and Human Breast Epithelial Cells Exhibit Differential TLR4 and TLR9 Signaling. Int. J. Mol. Sci. 2016, 17, 726.

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