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Int. J. Mol. Sci. 2016, 17(5), 710; doi:10.3390/ijms17050710

The Effect of Minimally Invasive Hematoma Aspiration on the JNK Signal Transduction Pathway after Experimental Intracerebral Hemorrhage in Rats

1
Department of Neurology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China
2
Department of Emergency, Linyi People’s Hospital, Linyi 276003, Shandong, China
3
Institute of Integrative Medicine, Qingdao University, Qingdao 266003, Shandong, China
4
Department of Radiology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China
5
Beijing Meidehoupu Science and Technology Company Limited, Beijing 102206, China
6
Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China
*
Author to whom correspondence should be addressed.
Academic Editor: Atsushi Matsuzawa
Received: 7 April 2016 / Revised: 29 April 2016 / Accepted: 6 May 2016 / Published: 13 May 2016
(This article belongs to the Special Issue Kinase Signal Transduction)
View Full-Text   |   Download PDF [3245 KB, uploaded 13 May 2016]   |  

Abstract

Objective: To explore the effect of minimally invasive hematoma aspiration (MIHA) on the c-Jun NH2-terminal kinase (JNK) signal transduction pathway after intracerebral hemorrhage (ICH). Methods: In this experiment, 300 adult male Wistar rats were randomly and averagely divided into sham-operated group, ICH group and MIHA group. In each group, 60 rats were used in the detection of indexes in this experiment, while the other 40 rats were used to replace rats which reached the exclusion criteria (accidental death or operation failure). In ICH group and MIHA group, ICH was induced by injection of 70 µL of autologous arterial blood into rat brain, while only the rats in MIHA group were treated by MIHA 6 h after ICH. Rats in sham-operated group were injected nothing into brains, and they were not treated either, like rats in ICH group. In each group, six rats were randomly selected to observe their Bederson’s scales persistently (6, 24, 48, 72, 96, 120 h after ICH). According to the time they were sacrificed, the remaining rats in each group were divided into 3 subgroups (24, 72, 120 h). The change of brain water content (BWC) was measured by the wet weight to dry weight ratio method. The morphology of neurons in cortex was observed by the hematoxylin–eosin (HE) staining. The expressions of phospho-c-Jun NH2-terminal kinase (pJNK) and JNK in peri-hematomal brain tissue were determined by the immunohistochemistry (IHC) and Western blotting (WB). Results: At all time points, compared with the ICH groups, the expression of pJNK decreased obviously in MIHA groups (p < 0.05), while their Bederson’s scales and BWC declined, and neuron injury in the cortex was relieved. The expression level of JNK was not altered at different groups. The data obtained by IHC and WB indicated a high-level of consistency, which provided a certain dependability of the test results. Conclusion: The JNK signal transduction pathway could be activated after intracerebral hemorrhage, with the expressions of pJNK increasing. MIHA could relieve the histo-pathological damage of nerve cells, reducing brain edema and neurological deficits, and these neuroprotective effects might be associated with suppression of JNK signal transduction pathway. View Full-Text
Keywords: minimally invasive hematoma aspiration; intracerebral hemorrhage; brain edema; JNK; rats minimally invasive hematoma aspiration; intracerebral hemorrhage; brain edema; JNK; rats
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MDPI and ACS Style

Pei, H.; Jiang, T.; Liu, G.; Li, Z.; Luo, K.; An, J.; Li, G.; Guo, Y. The Effect of Minimally Invasive Hematoma Aspiration on the JNK Signal Transduction Pathway after Experimental Intracerebral Hemorrhage in Rats. Int. J. Mol. Sci. 2016, 17, 710.

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