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Int. J. Mol. Sci. 2016, 17(5), 648; doi:10.3390/ijms17050648

Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster

1
Department of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto 606-8585, Japan
2
Department of Applied Biology, Kyoto Institute of Technology, Kyoto 606-8585, Japan
3
Department of Clinical Product Development, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki 852-8523, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Lee A. Bulla
Received: 29 March 2016 / Revised: 19 April 2016 / Accepted: 26 April 2016 / Published: 29 April 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Perilipins are evolutionarily conserved from Drosophila to humans, the lipid storage droplet 1 (Lsd1) is a Drosophila homolog of human perilipin 1. The function of Lsd1 as a regulator of lipolysis in Drosophila has been demonstrated, as the Lsd1 mutant causes an increase of lipid droplet size. However, the functions of this gene during development are still under investigation. In order to determine the function of Lsd1 during development, Lsd1 was knocked down in Drosophila using the GAL4-UAS system. Selective knockdown of Lsd1 in the dorsal wing disc caused an atrophied wing phenotype. The generation of reactive oxygen species in the wing pouch compartment of the Lsd1-knockdown flies was significantly higher than in the control. Immunostaining with caspase-3 antibody revealed a greater number of apoptotic cells in Lsd1-knockdown wing discs than in the control. Cell death by autophagy was also induced in the knockdown flies. Moreover, cells deprived of Lsd1 showed mitochondrial expansion and decreased ATP levels. These results strongly suggest that knockdown of Lsd1 induces mitochondrial stress and the production of reactive oxygen species that result in cell death, via apoptosis and the autophagy pathway. These results highlight the roles of Drosophila Lsd1 during wing development. View Full-Text
Keywords: Drosophila; lipid storage droplet 1; mitochondrial stress; perilipin; wing development Drosophila; lipid storage droplet 1; mitochondrial stress; perilipin; wing development
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MDPI and ACS Style

Men, T.T.; Binh, T.D.; Yamaguchi, M.; Huy, N.T.; Kamei, K. Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster. Int. J. Mol. Sci. 2016, 17, 648.

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