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Int. J. Mol. Sci. 2016, 17(3), 406; doi:10.3390/ijms17030406

Isolation and Expression Analysis of STAT Members from Synechogobius hasta and Their Roles in Leptin Affecting Lipid Metabolism

1
Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan 430070, China
2
Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China
*
Author to whom correspondence should be addressed.
Academic Editors: Jeffrey H. Boatright and Jun Li
Received: 11 February 2016 / Revised: 24 February 2016 / Accepted: 15 March 2016 / Published: 22 March 2016
(This article belongs to the Special Issue Fish Molecular Biology)
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Abstract

Signal transducers and activators of transcription proteins (STATs) act as important mediators in multiple biological processes induced by a large number of cytokines. In the present study, full-length cDNA sequences of seven STAT members, including some splicing variants different from those in mammals, were obtained from Synechogobius hasta. The phylogenetic analysis revealed that the seven STAT members were derived from paralogous genes that might have arisen by whole genome duplication (WGD) events during vertebrate evolution. All of these members share similar domain structure compared with those of mammals, and were widely expressed across the tested tissues (brain, gill, heart, intestine, liver, muscle and spleen), but at variable levels. Incubation in vitro of recombinant human leptin changed the intracellular triglyceride (TG) content and mRNA levels of several STATs members, as well as expressions and activities of genes involved in lipid metabolism. Furthermore, Tyrphostin B42 (AG490), a specific inhibitor of the Janus Kinase 2(JAK2)-STAT pathway, partially reversed leptin-induced change on STAT3 and its two spliced isoforms expression, as well as expressions and activities of genes involved in lipid metabolism. As a consequence, the decrease of TG content was also reversed. Thus, our study suggests that STAT3 is the requisite for the leptin signal and the activation of the STAT3 member may account for the leptin-induced changes in lipid metabolism in S. hasta. View Full-Text
Keywords: STATs; gene cloning; molecular characterization; leptin; lipid metabolism; Synechogobius hasta STATs; gene cloning; molecular characterization; leptin; lipid metabolism; Synechogobius hasta
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Wu, K.; Tan, X.-Y.; Wei, C.-C.; You, W.-J.; Zhuo, M.-Q.; Song, Y.-F. Isolation and Expression Analysis of STAT Members from Synechogobius hasta and Their Roles in Leptin Affecting Lipid Metabolism. Int. J. Mol. Sci. 2016, 17, 406.

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