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Int. J. Mol. Sci. 2016, 17(3), 345; doi:10.3390/ijms17030345

Molecular Characterization and Expression Analyses of the Complement Component C8α, C8β and C9 Genes in Yellow Catfish (Pelteobagrus fulvidraco) after the Aeromonas hydrophila Challenge

1
Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
2
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China
3
Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430070, China
4
School of Biological Sciences, Victoria University of Wellington, P O Box 600, Wellington 6140, New Zealand
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Li Lin
Received: 29 January 2016 / Revised: 26 February 2016 / Accepted: 29 February 2016 / Published: 8 March 2016
(This article belongs to the Special Issue Fish Molecular Biology)
View Full-Text   |   Download PDF [9222 KB, uploaded 8 March 2016]   |  

Abstract

The complement components C8α, C8β and C9 have important roles in the innate immune system against invading microorganisms. Partial cDNA sequences of the Pf_C8α, Pf_C8β and Pf_C9 genes (Pf: abbreviation of Pelteobagrus fulvidraco) were cloned from yellow catfish. The Pf_C8α, Pf_C8β and Pf_C9 genes showed the greatest amino acid similarity to C8α (54%) and C8β (62%) of zebrafish and to C9 (52%) of grass carp, respectively. Ontogenetic expression analyses using real-time quantitative PCR suggested that the three genes may play crucial roles during embryonic and early larval development. The mRNA expressions of the three genes were all at the highest levels in liver tissue, and at lower or much lower levels in 16 other tissues, demonstrating that the liver is the primary site for the protein synthesis of Pf_C8α, Pf_C8β and Pf_C9. Injection of Aeromonas hydrophila led to up-regulation of the three genes in the spleen, head kidney, kidney, liver and blood tissues, indicating that the three genes may contribute to the host’s defense against invading pathogenic microbes. An increased understanding of the functions of the Pf_C8α, Pf_C8β and Pf_C9 genes in the innate immunity of yellow catfish will help enhance production of this valuable freshwater species. View Full-Text
Keywords: Pelteobagrus fulvidraco; complement component; C8α; C8β; C9; cDNA cloning; gene expression; bacterial challenge Pelteobagrus fulvidraco; complement component; C8α; C8β; C9; cDNA cloning; gene expression; bacterial challenge
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Zheng, H.; Ji, W.; Zhang, G.-R.; Zhang, X.-T.; Shi, Z.-C.; Wei, K.-J.; Yang, R.-B.; Gardner, J.P.A. Molecular Characterization and Expression Analyses of the Complement Component C8α, C8β and C9 Genes in Yellow Catfish (Pelteobagrus fulvidraco) after the Aeromonas hydrophila Challenge. Int. J. Mol. Sci. 2016, 17, 345.

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