Next Article in Journal
Arginine Metabolism in Bacterial Pathogenesis and Cancer Therapy
Next Article in Special Issue
Arsenite Regulates Prolongation of Glycan Residues of Membrane Glycoprotein: A Pivotal Study via Wax Physisorption Kinetics and FTIR Imaging
Previous Article in Journal
Crosstalk between Long Noncoding RNAs and MicroRNAs in Health and Disease
Previous Article in Special Issue
Gleditsia sinensis Thorn Attenuates the Collagen-Based Migration of PC3 Prostate Cancer Cells through the Suppression of α2β1 Integrin Expression
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(3), 361; doi:10.3390/ijms17030361

Effects of Two Sublethal Concentrations of Mercury Chloride on the Morphology and Metallothionein Activity in the Liver of Zebrafish (Danio rerio)

1
Department of Biology, Ecology and Earth Science, University of Calabria, Via P. Bucci 4/B, Rende (Cosenza) 87036, Italy
2
Uni Research Environment, Uni Research, Bergen 5006, Norway
3
Department of Biology, University of Bergen, Bergen High Technology Center, Bergen 5020, Norway
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Received: 15 February 2016 / Revised: 2 March 2016 / Accepted: 4 March 2016 / Published: 11 March 2016
View Full-Text   |   Download PDF [9336 KB, uploaded 11 March 2016]   |  

Abstract

Mercury (Hg) is a highly hazardous pollutant widely used in industrial, pharmaceutical and agricultural fields. Mercury is found in the environment in several forms, elemental, inorganic (iHg) and organic, all of which are toxic. Considering that the liver is the organ primarily involved in the regulation of metabolic pathways, homeostasis and detoxification we investigated the morphological and ultrastructural effects in Danio rerio liver after 96 h exposure to two low HgCl2 concentrations (7.7 and 38.5 μg/L). We showed that a short-term exposure to very low concentrations of iHg severely affects liver morphology and ultrastructure. The main effects recorded in this work were: cytoplasm vacuolization, decrease in both lipid droplets and glycogen granules, increase in number of mitochondria, increase of rough endoplasmic reticulum and pyknotic nuclei. Pathological alterations observed were dose dependent. Trough immunohistochemistry, in situ hybridization and real-time PCR analysis, the induction of metallothionein (MT) under stressor conditions was also evaluated. Some of observed alterations could be considered as a general response of tissue to heavy metals, whereas others (such as increased number of mitochondria and increase of RER) may be considered as an adaptive response to mercury. View Full-Text
Keywords: inorganic mercury; liver; acute effects; histology; ultrastructure; confocal microscopy; in situ hybridization; metallothionein inorganic mercury; liver; acute effects; histology; ultrastructure; confocal microscopy; in situ hybridization; metallothionein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Macirella, R.; Guardia, A.; Pellegrino, D.; Bernabò, I.; Tronci, V.; Ebbesson, L.O.E.; Sesti, S.; Tripepi, S.; Brunelli, E. Effects of Two Sublethal Concentrations of Mercury Chloride on the Morphology and Metallothionein Activity in the Liver of Zebrafish (Danio rerio). Int. J. Mol. Sci. 2016, 17, 361.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top