Antithymocyte Globulin Induces a Tolerogenic Phenotype in Human Dendritic Cells
AbstractAntithymocyte globulin (ATG) is used in the prevention of graft-versus-host disease during allogeneic hematopoietic stem cell transplantation. It is generally accepted that ATG mediates its immunosuppressive effect primarily via depletion of T cells. Here, we analyzed the impact of ATG-Fresenius (now Grafalon®) on human monocyte-derived dendritic cells (DC). ATG induced a semi-mature phenotype in DC with significantly reduced expression of CD14, increased expression of HLA-DR, and intermediate expression of CD54, CD80, CD83, and CD86. ATG-DC showed an increase in IL-10 secretion but no IL-12 production. In line with this tolerogenic phenotype, ATG caused a significant induction of indoleamine 2,3-dioxygenase expression and a concomitant increase in levels of tryptophan metabolites in the supernatants of DC. Further, ATG-DC did not induce the proliferation of allogeneic T cells in a mixed lymphocyte reaction but actively suppressed the T cell proliferation induced by mature DC. These data suggest that besides its well-known effect on T cells, ATG modulates the phenotype of DC in a tolerogenic way, which might constitute an essential part of its immunosuppressive action in vivo. View Full-Text
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Roider, T.; Katzfuß, M.; Matos, C.; Singer, K.; Renner, K.; Oefner, P.J.; Dettmer-Wilde, K.; Herr, W.; Holler, E.; Kreutz, M.; Peter, K. Antithymocyte Globulin Induces a Tolerogenic Phenotype in Human Dendritic Cells. Int. J. Mol. Sci. 2016, 17, 2081.
Roider T, Katzfuß M, Matos C, Singer K, Renner K, Oefner PJ, Dettmer-Wilde K, Herr W, Holler E, Kreutz M, Peter K. Antithymocyte Globulin Induces a Tolerogenic Phenotype in Human Dendritic Cells. International Journal of Molecular Sciences. 2016; 17(12):2081.Chicago/Turabian Style
Roider, Tobias; Katzfuß, Michael; Matos, Carina; Singer, Katrin; Renner, Kathrin; Oefner, Peter J.; Dettmer-Wilde, Katja; Herr, Wolfgang; Holler, Ernst; Kreutz, Marina; Peter, Katrin. 2016. "Antithymocyte Globulin Induces a Tolerogenic Phenotype in Human Dendritic Cells." Int. J. Mol. Sci. 17, no. 12: 2081.
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