Next Article in Journal
Pathophysiology of Non Alcoholic Fatty Liver Disease
Next Article in Special Issue
Advances in Monitoring Cell-Based Therapies with Magnetic Resonance Imaging: Future Perspectives
Previous Article in Journal
Oxidative Stress in Hypoxic-Ischemic Encephalopathy: Molecular Mechanisms and Therapeutic Strategies
Previous Article in Special Issue
Checkpoints to the Brain: Directing Myeloid Cell Migration to the Central Nervous System
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(12), 2081; doi:10.3390/ijms17122081

Antithymocyte Globulin Induces a Tolerogenic Phenotype in Human Dendritic Cells

1
Department of Internal Medicine III, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany
2
Institute of Functional Genomics, University of Regensburg, Am BioPark 9, 93053 Regensburg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Maurizio Muraca
Received: 18 August 2016 / Revised: 23 November 2016 / Accepted: 5 December 2016 / Published: 11 December 2016
(This article belongs to the Special Issue Advances in Cell Transplantation)
View Full-Text   |   Download PDF [1740 KB, uploaded 12 December 2016]   |  

Abstract

Antithymocyte globulin (ATG) is used in the prevention of graft-versus-host disease during allogeneic hematopoietic stem cell transplantation. It is generally accepted that ATG mediates its immunosuppressive effect primarily via depletion of T cells. Here, we analyzed the impact of ATG-Fresenius (now Grafalon®) on human monocyte-derived dendritic cells (DC). ATG induced a semi-mature phenotype in DC with significantly reduced expression of CD14, increased expression of HLA-DR, and intermediate expression of CD54, CD80, CD83, and CD86. ATG-DC showed an increase in IL-10 secretion but no IL-12 production. In line with this tolerogenic phenotype, ATG caused a significant induction of indoleamine 2,3-dioxygenase expression and a concomitant increase in levels of tryptophan metabolites in the supernatants of DC. Further, ATG-DC did not induce the proliferation of allogeneic T cells in a mixed lymphocyte reaction but actively suppressed the T cell proliferation induced by mature DC. These data suggest that besides its well-known effect on T cells, ATG modulates the phenotype of DC in a tolerogenic way, which might constitute an essential part of its immunosuppressive action in vivo. View Full-Text
Keywords: allogeneic hematopoietic stem cell transplantation; dendritic cell; antithymocyte globulin; Grafalon; tolerogenic; indoleamine 2,3-dioxygenase; immunosuppressive allogeneic hematopoietic stem cell transplantation; dendritic cell; antithymocyte globulin; Grafalon; tolerogenic; indoleamine 2,3-dioxygenase; immunosuppressive
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Roider, T.; Katzfuß, M.; Matos, C.; Singer, K.; Renner, K.; Oefner, P.J.; Dettmer-Wilde, K.; Herr, W.; Holler, E.; Kreutz, M.; Peter, K. Antithymocyte Globulin Induces a Tolerogenic Phenotype in Human Dendritic Cells. Int. J. Mol. Sci. 2016, 17, 2081.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top