Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells
AbstractChloranthalactone B (CTB), a lindenane-type sesquiterpenoid, was obtained from the Chinese medicinal herb Sarcandra glabra, which is frequently used as a remedy for inflammatory diseases. However, the anti-inflammatory mechanisms of CTB have not been fully elucidated. In this study, we investigated the molecular mechanisms underlying these effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. CTB strongly inhibited the production of nitric oxide and pro-inflammatory mediators such as prostaglandin E2, tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 in RAW264.7 cells stimulated with LPS. A reverse-transcription polymerase chain reaction assay and Western blot further confirmed that CTB inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, and IL-1β at the transcriptional level, and decreased the luciferase activities of activator protein (AP)-1 reporter promoters. These data suggest that inhibition occurred at the transcriptional level. In addition, CTB blocked the activation of p38 mitogen-activated protein kinase (MAPK) but not c-Jun N-terminal kinase or extracellular signal-regulated kinase 1/2. Furthermore, CTB suppressed the phosphorylation of MKK3/6 by targeting the binding sites via formation of hydrogen bonds. Our findings clearly show that CTB inhibits the production of inflammatory mediators by inhibiting the AP-1 and p38 MAPK pathways. Therefore, CTB could potentially be used as an anti-inflammatory agent. View Full-Text
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Li, X.; Shen, J.; Jiang, Y.; Shen, T.; You, L.; Sun, X.; Xu, X.; Hu, W.; Wu, H.; Wang, G. Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells. Int. J. Mol. Sci. 2016, 17, 1938.
Li X, Shen J, Jiang Y, Shen T, You L, Sun X, Xu X, Hu W, Wu H, Wang G. Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells. International Journal of Molecular Sciences. 2016; 17(11):1938.Chicago/Turabian Style
Li, Xueqin; Shen, Jun; Jiang, Yunyao; Shen, Ting; You, Long; Sun, Xiaobo; Xu, Xudong; Hu, Weicheng; Wu, Haifeng; Wang, Gongcheng. 2016. "Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells." Int. J. Mol. Sci. 17, no. 11: 1938.
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