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Int. J. Mol. Sci. 2016, 17(11), 1938; doi:10.3390/ijms17111938

Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells

1,†
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2,†
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3,4,†
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5,8
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5,8
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6
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6
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5,8,* , 6,* and 7,*
1
Department of Gerontology, Huai’an First People’s Hospital, Nanjing Medical University, Huaian 223300, China
2
Department of Neurology, Huai’an Hospital Affiliated of Xuzhou Medical College and Huai’an Second People’s Hospital, Huaian 223300, China
3
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
4
Shineway Pharmaceutical Group Co., Ltd., Shijiazhuang 051430, China
5
Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huaian 223300, China
6
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China
7
Department of Urology, Huai’an First People’s Hospital, Nanjing Medical University, 6 Beijing West Road, Huaian 223300, China
8
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Chang Won Choi
Received: 30 September 2016 / Revised: 8 November 2016 / Accepted: 11 November 2016 / Published: 22 November 2016
(This article belongs to the Section Bioactives and Nutraceuticals)
View Full-Text   |   Download PDF [1495 KB, uploaded 22 November 2016]   |  

Abstract

Chloranthalactone B (CTB), a lindenane-type sesquiterpenoid, was obtained from the Chinese medicinal herb Sarcandra glabra, which is frequently used as a remedy for inflammatory diseases. However, the anti-inflammatory mechanisms of CTB have not been fully elucidated. In this study, we investigated the molecular mechanisms underlying these effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. CTB strongly inhibited the production of nitric oxide and pro-inflammatory mediators such as prostaglandin E2, tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 in RAW264.7 cells stimulated with LPS. A reverse-transcription polymerase chain reaction assay and Western blot further confirmed that CTB inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, and IL-1β at the transcriptional level, and decreased the luciferase activities of activator protein (AP)-1 reporter promoters. These data suggest that inhibition occurred at the transcriptional level. In addition, CTB blocked the activation of p38 mitogen-activated protein kinase (MAPK) but not c-Jun N-terminal kinase or extracellular signal-regulated kinase 1/2. Furthermore, CTB suppressed the phosphorylation of MKK3/6 by targeting the binding sites via formation of hydrogen bonds. Our findings clearly show that CTB inhibits the production of inflammatory mediators by inhibiting the AP-1 and p38 MAPK pathways. Therefore, CTB could potentially be used as an anti-inflammatory agent. View Full-Text
Keywords: Sarcandra glabra; sesquiterpene; chloranthalactone B; inflammation Sarcandra glabra; sesquiterpene; chloranthalactone B; inflammation
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MDPI and ACS Style

Li, X.; Shen, J.; Jiang, Y.; Shen, T.; You, L.; Sun, X.; Xu, X.; Hu, W.; Wu, H.; Wang, G. Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells. Int. J. Mol. Sci. 2016, 17, 1938.

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