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Int. J. Mol. Sci. 2016, 17(10), 1677; doi:10.3390/ijms17101677

Identification and Functional Analysis of microRNAs Involved in the Anther Development in Cotton Genic Male Sterile Line Yu98-8A

1
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
2
Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA
3
State Key Laboratory of Cotton Biology, Cotton Research Institute, CAAS, Anyang 455000, China
4
Handan Agronomy of Agricultural Sciences, Handan 056006, China
5
Agronomy College, Henan Agricultural University, Zhengzhou 450002, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 7 July 2016 / Revised: 22 September 2016 / Accepted: 23 September 2016 / Published: 7 October 2016
(This article belongs to the Section Molecular Botany)
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Abstract

Hybrid vigor contributes in a large way to the yield and quality of cotton (Gossypium hirsutum) fiber. Although microRNAs play essential regulatory roles in flower induction and development, it is still unclear if microRNAs are involved in male sterility, as the regulatory molecular mechanisms of male sterility in cotton need to be better defined. In this study, two independent small RNA libraries were constructed and sequenced from the young buds collected from the sporogenous cell formation to the meiosis stage of the male sterile line Yu98-8A and the near-isogenic line. Sequencing revealed 1588 and 1536 known microRNAs and 347 and 351 novel miRNAs from male sterile and male fertile libraries, respectively. MicroRNA expression profiles revealed that 49 conserved and 51 novel miRNAs were differentially expressed. Bioinformatic and degradome analysis indicated the regulatory complexity of microRNAs during flower induction and development. Further RT-qPCR and physiological analysis indicated that, among the different Kyoto Encyclopedia Gene and Genomes pathways, indole-3-acetic acid and gibberellic acid signaling transduction pathways may play pivotal regulatory functions in male sterility. View Full-Text
Keywords: cotton; male abortion; miRNAs; targets; molecular regulation; phytohormone cotton; male abortion; miRNAs; targets; molecular regulation; phytohormone
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Yang, X.; Zhao, Y.; Xie, D.; Sun, Y.; Zhu, X.; Esmaeili, N.; Yang, Z.; Wang, Y.; Yin, G.; Lv, S.; Nie, L.; Tang, Z.; Zhao, F.; Li, W.; Mishra, N.; Sun, L.; Zhu, W.; Fang, W. Identification and Functional Analysis of microRNAs Involved in the Anther Development in Cotton Genic Male Sterile Line Yu98-8A. Int. J. Mol. Sci. 2016, 17, 1677.

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