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Int. J. Mol. Sci. 2016, 17(10), 1617; doi:10.3390/ijms17101617

Long Noncoding RNA lncCAMTA1 Promotes Proliferation and Cancer Stem Cell-Like Properties of Liver Cancer by Inhibiting CAMTA1

1
Department of Radio-Oncology, First Hospital of Jilin University, No. 71 Xinmin Street, Changchun 130021, China
2
Academy of Laboratory, Jilin Medical University, No. 5 Jilin Street, Jilin 132013, China
3
Center of Cardiovascular Diseases, First Hospital of Jilin University, No. 71 Xinmin Street, Changchun 130021, China
4
Department of Interventional Therapy, First Hospital of Jilin University, No. 71 Xinmin Street, Changchun 130021, China
5
Department of Pathology, Jilin Medical University, No. 5 Jilin Street, Jilin 132013, China
6
Department of Hepatobiliary and Pancreas Surgery, First Hospital of Jilin University, No. 71 Xinmin Street, Changchun 130021, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Martin Pichler
Received: 7 July 2016 / Revised: 3 September 2016 / Accepted: 5 September 2016 / Published: 23 September 2016
(This article belongs to the Collection Regulation by Non-Coding RNAs)
View Full-Text   |   Download PDF [4057 KB, uploaded 23 September 2016]   |  

Abstract

Hepatocellular carcinoma (HCC) is the most common subtype of liver malignancy, and it is characterized by poor prognosis because of cancer stem cell (CSC)-mediated high postsurgical recurrence rates. Thus, targeting CSCs, or HCC cells with CSC-like properties, is an effective strategy for HCC therapy. Here, using long noncoding RNA (lncRNA) microarray analysis, we identified a novel lncRNA termed lncCAMTA1 that is increased in both liver CSCs and HCC. High lncCAMTA1 expression in HCC indicates poor clinical outcome. In vitro and in vivo functional experiments showed that overexpression of lncCAMTA1 promotes HCC cell proliferation, CSC-like properties, and tumorigenesis. Conversely, depletion of lncCAMTA1 inhibits HCC cell proliferation, CSC-like properties, and tumorigenesis. Mechanistically, we demonstrated that lncCAMTA1 physically associates with the calmodulin binding transcription activator 1 (CAMTA1) promoter, induces a repressive chromatin structure, and inhibits CAMTA1 transcription. Furthermore, CAMTA1 is required for the effects of lncCAMTA1 on HCC cell proliferation and CSC-like properties, and the expression of lncCAMTA1 and CAMTA1 is significantly negatively correlated in HCC tissues. Collectively, our study revealed the important roles and underlying molecular mechanisms of lncCAMTA1 on HCC, and suggested that lncCAMTA1 could be an effective prognostic factor and a potential therapeutic target for HCC. View Full-Text
Keywords: long noncoding RNA; cancer stem cells; liver cancer; tumorigenesis; CAMTA1 long noncoding RNA; cancer stem cells; liver cancer; tumorigenesis; CAMTA1
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Ding, L.-J.; Li, Y.; Wang, S.-D.; Wang, X.-S.; Fang, F.; Wang, W.-Y.; Lv, P.; Zhao, D.-H.; Wei, F.; Qi, L. Long Noncoding RNA lncCAMTA1 Promotes Proliferation and Cancer Stem Cell-Like Properties of Liver Cancer by Inhibiting CAMTA1. Int. J. Mol. Sci. 2016, 17, 1617.

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