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Int. J. Mol. Sci. 2016, 17(10), 1493; doi:10.3390/ijms17101493

MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer

1,†
,
1,2,†
and
1,*
1
Laboratory of Molecular and Experimental Pathology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
2
Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650223, China
*
Author to whom correspondence should be addressed.
Academic Editors: Y-h. Taguchi and Kumiko UI-TEI
Received: 25 July 2016 / Revised: 29 August 2016 / Accepted: 30 August 2016 / Published: 26 September 2016
(This article belongs to the Special Issue microRNA Regulation 2017)
View Full-Text   |   Download PDF [2770 KB, uploaded 28 September 2016]   |  

Abstract

MicroRNAs (miRNAs) have critical roles in lung tumorigenesis and development. To determine aberrantly expressed miRNAs involved in non-small cell lung cancer (NSCLC) and investigate pathophysiological functions and mechanisms, we firstly carried out small RNA deep sequencing in NSCLC cell lines (EPLC-32M1, A549 and 801D) and a human immortalized cell line 16HBE, we then studied miRNA function by cell proliferation and apoptosis. cDNA microarray, luciferase reporter assay and miRNA transfection were used to investigate interaction between the miRNA and target gene. miR-944 was significantly down-regulated in NSCLC and had many putative targets. Moreover, the forced expression of miR-944 significantly inhibited the proliferation of NSCLC cells in vitro. By integrating mRNA expression data and miR-944-target prediction, we disclosed that EPHA7 was a potential target of miR-944, which was further verified by luciferase reporter assay and microRNA transfection. Our data indicated that miR-944 targets EPHA7 in NSCLC and regulates NSCLC cell proliferation, which may offer a new mechanism underlying the development and progression of NSCLC. View Full-Text
Keywords: non-small cell lung cancer (NSCLC); miR-944; regulation; cell proliferation; EPHA7 non-small cell lung cancer (NSCLC); miR-944; regulation; cell proliferation; EPHA7
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Liu, M.; Zhou, K.; Cao, Y. MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2016, 17, 1493.

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