MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer
Abstract
:1. Introduction
2. Results
2.1. The Cancer-Related miRNAs Were Screened in Cultured NSCLC Cells
2.2. The miR-944 Expression Was Down-Regulated in NSCLC Tissues and Cell Lines
2.3. The Overexpression of miR-944 Could Suppress the Proliferation of NSCLC Cells
2.4. EPHA7 (a Target of miR-944) Was Screened in NSCLCs
2.5. The Expression Level of EPHA7 Is Inversely Correlated with the miR-944 Expression in NSCLC
2.6. The Up-Regulation of miR-944 Could Suppress the EPHA7 Expression
2.7. miR-944 Directly Targeted EPHA7 by Interacting with Its 3′-Untranslated Regions (UTRs)
2.8. The Down-Regulation of EPHA7 Could Suppress the Proliferation of NSCLC Cells
3. Discussion
4. Experimental Section
4.1. Human Tissue Specimens and Cell Lines
4.2. miRNA Expression Profiling
4.3. miRNA Target Prediction
4.4. Quantitative Real-Time PCR (qRT-PCR)
4.5. Oligonucleotide Transfection
4.6. MRNA Expression Profiling
4.7. Western Blot
4.8. Cell Growth and Apoptosis Assay
4.9. Luciferase Reporter Assays
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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miRNA | Log2(Fold Change) | Cytoband | ||
---|---|---|---|---|
EPLC-32M1 vs. 16HBE | 801D vs. 16HBE | A549 vs. 16HBE | ||
Up-Regulated miRNAs | ||||
hsa-miR-10a-3p | 4.446 | 6.715 | 4.289 | 17q21.32 |
hsa-miR-10a-5p | 5.816 | 8.581 | 4.626 | 17q21.32 |
hsa-miR-10b-5p | 8.912 | 6.686 | 8.627 | 17q21.32 |
hsa-miR-137 | 8.775 | 9.571 | 8.524 | 1p22 |
hsa-miR-140-3p | 4.144 | 4.659 | 3.415 | 16q22.1 |
hsa-miR-193a-5p | 4.103 | 6.348 | 4.054 | 17q11.2 |
hsa-miR-224-5p | 13.982 | 9.493 | 12.369 | Xq28 |
hsa-miR-2682-5p | 8.822 | 10.672 | 8.441 | Chromosome1: (98510798-98510907) |
hsa-miR-31-3p | 3.050 | 4.505 | 5.289 | 9p21.3 |
hsa-miR-3179 | 7.568 | 8.893 | 7.469 | 16p13.11 |
hsa-miR-320d | 5.557 | 3.675 | 3.404 | 13q14.11 |
hsa-miR-365b-5p | 5.031 | 7.127 | 6.003 | 17q11.2 |
hsa-miR-452-5p | 13.175 | 7.857 | 10.770 | Xq28 |
hsa-miR-935 | 7.900 | 10.634 | 7.664 | 19q13.42 |
Down-Regulated miRNAs | ||||
hsa-miR-129-1-3p | 9.110 | 3.802 | 6.340 | 7q32 |
hsa-miR-141-3p | 6.884 | 11.752 | 7.398 | 12p13.31 |
hsa-miR-200c-3p | 7.068 | 9.662 | 7.691 | 12p13.31 |
hsa-miR-203a | 4.007 | 3.724 | 9.962 | 14q32.33 |
hsa-miR-205-3p | 6.766 | 6.766 | 6.766 | 1q32.2 |
hsa-miR-544b | 6.650 | 6.650 | 6.6501 | Chromosome3: (124451286-124451363) |
hsa-miR-934 | 8.313 | 8.313 | 8.313 | Xq26.3 |
hsa-miR-936 | 7.650 | 7.650 | 7.650 | 10q25.1 |
hsa-miR-944 | 3.597 | 7.235 | 3.4975 | 3q28 |
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Liu, M.; Zhou, K.; Cao, Y. MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2016, 17, 1493. https://doi.org/10.3390/ijms17101493
Liu M, Zhou K, Cao Y. MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer. International Journal of Molecular Sciences. 2016; 17(10):1493. https://doi.org/10.3390/ijms17101493
Chicago/Turabian StyleLiu, Minxia, Kecheng Zhou, and Yi Cao. 2016. "MicroRNA-944 Affects Cell Growth by Targeting EPHA7 in Non-Small Cell Lung Cancer" International Journal of Molecular Sciences 17, no. 10: 1493. https://doi.org/10.3390/ijms17101493