Biliverdin Reductase A (BVRA) Mediates Macrophage Expression of Interleukin-10 in Injured Kidney
Abstract
:1. Introduction
2. Results
2.1. M1 and M2 Macrophage Polarization of Bone Marrow Derived Macrophage was Induced by GM-CSF and M-CSF
2.2. BVRA Expression Increased in M2 macrophage, Compared to M1 macrophage
2.3. BVRA Mediated IL-10 Expression in Macrophages
2.4. BVRA Positive Macrophages Existed in Vivo Renal Tissue.
2.5. GM-CSF, M-CSF or LPS Increased IL-10 Expression in BVRA Positive Macrophages
3. Discussion
4. Experimental Section
4.1. Mice
4.2. Renal Ischemia Reperfusion Injury
4.3. Cell Culture
4.3.1. L929 Cells
4.3.2. Preparation for Bone Marrow Derived Macrophages (BMDM)
4.4. Recombinant Adenoviruses Transfection
4.5. Recombinant Lentivirus Transfection
4.6. Western Blotting
4.7. Quantitative RT-PCR
Gene Names | Primer Sequence |
---|---|
BVRA | Qiagen, Germany, QT00118762 |
ARG-1 | Qiagen, Germany, QT00134288 |
IL-10 | Qiagen, Germany, QT00106169 |
INOS | Qiagen, Germany, QT01547980 |
TNF-α | Qiagen, Germany, QT00104006 |
GAPDH | Qiagen, Germany, QT01658692 |
MMR(CD206) | Qiagen, Germany, QT00103012 |
4.8. Flow Cytometry
4.9. Elisa Assay
4.10. Immunofluorescence
4.11. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Hu, Z.; Pei, G.; Wang, P.; Yang, J.; Zhu, F.; Guo, Y.; Wang, M.; Yao, Y.; Zeng, R.; Liao, W.; et al. Biliverdin Reductase A (BVRA) Mediates Macrophage Expression of Interleukin-10 in Injured Kidney. Int. J. Mol. Sci. 2015, 16, 22621-22635. https://doi.org/10.3390/ijms160922621
Hu Z, Pei G, Wang P, Yang J, Zhu F, Guo Y, Wang M, Yao Y, Zeng R, Liao W, et al. Biliverdin Reductase A (BVRA) Mediates Macrophage Expression of Interleukin-10 in Injured Kidney. International Journal of Molecular Sciences. 2015; 16(9):22621-22635. https://doi.org/10.3390/ijms160922621
Chicago/Turabian StyleHu, Zhizhi, Guangchang Pei, Pengge Wang, Juan Yang, Fengmin Zhu, Yujiao Guo, Meng Wang, Ying Yao, Rui Zeng, Wenhui Liao, and et al. 2015. "Biliverdin Reductase A (BVRA) Mediates Macrophage Expression of Interleukin-10 in Injured Kidney" International Journal of Molecular Sciences 16, no. 9: 22621-22635. https://doi.org/10.3390/ijms160922621