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Int. J. Mol. Sci. 2015, 16(9), 22606-22620; doi:10.3390/ijms160922606

Identification and Characterization of CYP9A40 from the Tobacco Cutworm Moth (Spodoptera litura), a Cytochrome P450 Gene Induced by Plant Allelochemicals and Insecticides

1
Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China
2
Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China
3
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University (East Campus), Guangzhou 510006, China
4
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
Author to whom correspondence should be addressed.
Academic Editor: Lee A. Bulla
Received: 14 August 2015 / Revised: 6 September 2015 / Accepted: 11 September 2015 / Published: 18 September 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [1728 KB, uploaded 18 September 2015]   |  

Abstract

Cytochrome P450 monooxygenases (P450s) of insects play crucial roles in the metabolism of endogenous and dietary compounds. Tobacco cutworm moth (Spodoptera litura), an important agricultural pest, causes severe yield losses in many crops. In this study, we identified CYP9A40, a novel P450 gene of S. litura, and investigated its expression profile and potential role in detoxification of plant allelochemicals and insecticides. The cDNA contains an open reading frame encoding 529 amino acid residues. CYP9A40 transcripts were found to be accumulated during various development stages of S. litura and were highest in fifth and sixth instar larvae. CYP9A40 was mainly expressed in the midgut and fat body. Larval consumption of xenobiotics, namely plant allelochemicals (quercetin and cinnamic acid) and insecticides (deltamethrin and methoxyfenozide) induced accumulation of CYP9A40 transcripts in the midgut and fat body. Injection of dsCYP9A40 (silencing of CYP9A40 by RNA interference) significantly increased the susceptibility of S. litura larvae to the tested plant allelochemicals and insecticides. These results indicate that CYP9A40 expression in S. litura is related to consumption of xenobiotics and suggest that CYP9A40 is involved in detoxification of these compounds. View Full-Text
Keywords: Spodoptera litura; cytochrome P450 monooxygenase; RNA interference; insecticide detoxification; plant allelochemicals Spodoptera litura; cytochrome P450 monooxygenase; RNA interference; insecticide detoxification; plant allelochemicals
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wang, R.-L.; Staehelin, C.; Xia, Q.-Q.; Su, Y.-J.; Zeng, R.-S. Identification and Characterization of CYP9A40 from the Tobacco Cutworm Moth (Spodoptera litura), a Cytochrome P450 Gene Induced by Plant Allelochemicals and Insecticides. Int. J. Mol. Sci. 2015, 16, 22606-22620.

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