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Int. J. Mol. Sci. 2015, 16(9), 20290-20307; doi:10.3390/ijms160920290

Rapid Bioassay-Guided Isolation of Antibacterial Clerodane Type Diterpenoid from Dodonaea viscosa (L.) Jaeq.

1
Department of Pharmacy, Shaheed Benazir Bhutto University, Sheringal, Dir Upper 18000, Pakistan
2
School of Pharmacy and Life Sciences, the Robert Gordon University, Aberdeen AB25 1HG, UK
3
Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan
4
Department of Agricultural and Environmental Sciences, Milan State University, Milan 20133, Italy
5
Centre for Interdisciplinary Research in Basic Sciences, International Islamic University, Islamabad 44000, Pakistan
6
Department of Chemistry, Kohat University of Science & Technology, Kohat 26000, Pakistan
7
Department of Chemistry, Shaheed Benazir Bhutto University, Sheringal, Dir Upper 18000, Pakistan
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Chang Won Choi
Received: 6 March 2015 / Revised: 14 August 2015 / Accepted: 17 August 2015 / Published: 27 August 2015
(This article belongs to the Special Issue Molecular Research in Plant Secondary Metabolism 2015)
View Full-Text   |   Download PDF [1679 KB, uploaded 27 August 2015]   |  

Abstract

Plant extracts are complex matrices and, although crude extracts are widely in use, purified compounds are pivotal in drug discovery. This study describes the application of automated preparative-HPLC combined with a rapid off-line bacterial bioassay, using reduction of a tetrazolium salt as an indicator of bacterial metabolism. This approach enabled the identification of fractions from Dodonaea viscosa that were active against Staphylococcus aureus and Escherichia coli, which, ultimately, resulted in the identification of a clerodane type diterpenoid, 6β-hydroxy-15,16-epoxy-5β, 8β, 9β, 10α-cleroda-3, 13(16), 14-trien-18-oic acid, showing bacteriostatic activity (minimum inhibitory concentration (MIC) = 64–128 µg/mL) against test bacteria. To the best of our knowledge, this is the first report on antibacterial activity of this metabolite from D. viscosa. View Full-Text
Keywords: Dodonaea viscosa; preparative HPLC; XTT assay; clerodane diterpenoid Dodonaea viscosa; preparative HPLC; XTT assay; clerodane diterpenoid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Khurram, M.; Lawton, L.A.; Edwards, C.; Iriti, M.; Hameed, A.; Khan, M.A.; Khan, F.A.; Rahman, S.U. Rapid Bioassay-Guided Isolation of Antibacterial Clerodane Type Diterpenoid from Dodonaea viscosa (L.) Jaeq.. Int. J. Mol. Sci. 2015, 16, 20290-20307.

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