Next Article in Journal
Dual Inhibition of MEK and PI3K Pathway in KRAS and BRAF Mutated Colorectal Cancers
Next Article in Special Issue
Constituents and Pharmacological Activities of Myrcia (Myrtaceae): A Review of an Aromatic and Medicinal Group of Plants
Previous Article in Journal
Zhou, N., et al. Exposure of Tumor-Associated Macrophages to ApoptoticMCF-7 Cells Promotes Breast Cancer Growth and Metastasis. Int. J. Mol. Sci. 2015, 16, 11966–11982
Previous Article in Special Issue
Rapid Bioassay-Guided Isolation of Antibacterial Clerodane Type Diterpenoid from Dodonaea viscosa (L.) Jaeq.
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(9), 22960-22975; doi:10.3390/ijms160922960

Exogenous GA3 Application Enhances Xylem Development and Induces the Expression of Secondary Wall Biosynthesis Related Genes in Betula platyphylla

1
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
2
Department of Life Science and Technology, Mudanjiang Normal College, Mudanjiang 157012, China
3
Forestry Science Research Institute of Heilongjiang Province, Harbin 150081, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 18 May 2015 / Revised: 9 September 2015 / Accepted: 11 September 2015 / Published: 23 September 2015
(This article belongs to the Special Issue Molecular Research in Plant Secondary Metabolism 2015)
View Full-Text   |   Download PDF [2896 KB, uploaded 23 September 2015]   |  

Abstract

Gibberellin (GA) is a key signal molecule inducing differentiation of tracheary elements, fibers, and xylogenesis. However the molecular mechanisms underlying the effect of GA on xylem elongation and secondary wall development in tree species remain to be determined. In this study, Betula platyphylla (birch) seeds were treated with 300 ppm GA3 and/or 300 ppm paclobutrazol (PAC), seed germination was recorded, and transverse sections of hypocotyls were stained with toluidine blue; the two-month-old seedlings were treated with 50 μM GA3 and/or 50 μM PAC, transverse sections of seedling stems were stained using phloroglucinol–HCl, and secondary wall biosynthesis related genes expression was analyzed by real-time quantitative PCR. Results indicated that germination percentage, energy and time of seeds, hypocotyl height and seedling fresh weight were enhanced by GA3, and reduced by PAC; the xylem development was wider in GA3-treated plants than in the control; the expression of NAC and MYB transcription factors, CESA, PAL, and GA oxidase was up-regulated during GA3 treatment, suggesting their role in GA3-induced xylem development in the birch. Our results suggest that GA3 induces the expression of secondary wall biosynthesis related genes to trigger xylogenesis in the birch plants. View Full-Text
Keywords: Betula platyphylla SUK; GA3; seed germination; xylem development; secondary wall biosynthesis related genes Betula platyphylla SUK; GA3; seed germination; xylem development; secondary wall biosynthesis related genes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Guo, H.; Wang, Y.; Liu, H.; Hu, P.; Jia, Y.; Zhang, C.; Wang, Y.; Gu, S.; Yang, C.; Wang, C. Exogenous GA3 Application Enhances Xylem Development and Induces the Expression of Secondary Wall Biosynthesis Related Genes in Betula platyphylla. Int. J. Mol. Sci. 2015, 16, 22960-22975.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top