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Int. J. Mol. Sci. 2015, 16(8), 18507-18521; doi:10.3390/ijms160818507

Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

1
Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Keelung 204, Taiwan
2
College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
3
Bone and Joint Research Center, Department of Orthopedics and Traumatology, Taipei Medical University Hospital, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan
4
Spine Department, Lin-Sun Hospital, Taichung 403, Taiwan
5
Division of Pediatric General Medicine, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan
6
Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Mohamed N. Rahaman
Received: 22 January 2015 / Revised: 21 July 2015 / Accepted: 31 July 2015 / Published: 7 August 2015
(This article belongs to the Special Issue Biomaterials for Tissue Engineering)
View Full-Text   |   Download PDF [4472 KB, uploaded 7 August 2015]   |  

Abstract

This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50% PRP concentration yielded better differentiation than the 20% PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries. View Full-Text
Keywords: platelet-rich plasma; synovial fluid mesenchymal stem cells; pigs; alginate hydrogel; gene expressions; DNA proliferation; chondrogenesis platelet-rich plasma; synovial fluid mesenchymal stem cells; pigs; alginate hydrogel; gene expressions; DNA proliferation; chondrogenesis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tang, H.-C.; Chen, W.-C.; Chiang, C.-W.; Chen, L.-Y.; Chang, Y.-C.; Chen, C.-H. Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel. Int. J. Mol. Sci. 2015, 16, 18507-18521.

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