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Int. J. Mol. Sci. 2015, 16(8), 18796-18811; doi:10.3390/ijms160818796

Influence of Pre-Freezing Temperature on the Corneal Endothelial Cytocompatibility and Cell Delivery Performance of Porous Hyaluronic Acid Hydrogel Carriers

1,2,3,4
1
Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan
2
Biomedical Engineering Research Center, Chang Gung University, Taoyuan 33302, Taiwan
3
Molecular Medicine Research Center, Chang Gung University, Taoyuan 33302, Taiwan
4
Center for Tissue Engineering, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan 
Academic Editor: Mohamed N. Rahaman
Received: 2 May 2015 / Revised: 21 July 2015 / Accepted: 31 July 2015 / Published: 11 August 2015
(This article belongs to the Special Issue Biomaterials for Tissue Engineering)
View Full-Text   |   Download PDF [1981 KB, uploaded 11 August 2015]   |  

Abstract

The development of porous hyaluronic acid (HA) hydrogels for corneal endothelial tissue engineering is attractive because they can be used as functional cell delivery carriers to help in the reconstruction of damaged areas. The purpose of this study was to investigate the corneal endothelial cytocompatibility and cell delivery performance of porous HA hydrogel biomaterials fabricated at different pre-freezing temperatures. As compared to their counterparts prepared at −80 °C, the HA samples fabricated at higher pre-freezing temperature (i.e., 0 °C) exhibited a larger pore size and higher porosity, thereby leading to lower resistance to glucose permeation. Live/dead assays and gene expression analyses showed that the restricted porous structure of HA carriers decreases the viability and ionic pump function of cultured corneal endothelial cells (CECs). The results also indicated that the porous hydrogel biomaterials fabricated at high pre-freezing temperature seem to be more compatible with rabbit CECs. In an animal model of corneal endothelial dysfunction, the wounded rabbit corneas receiving bioengineered CEC sheets and restricted porous-structured HA carriers demonstrated poor tissue reconstruction. The therapeutic efficacy of cell sheet transplants can be improved by using carrier materials prepared at high pre-freezing temperature. Our findings suggest that the cryogenic operation temperature-mediated pore microstructure of HA carriers plays an important role in corneal endothelial cytocompatibility and cell delivery performance. View Full-Text
Keywords: hyaluronic acid; pre-freezing temperature; porous hydrogel carrier; corneal endothelial cytocompatibility; cell delivery performance hyaluronic acid; pre-freezing temperature; porous hydrogel carrier; corneal endothelial cytocompatibility; cell delivery performance
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lai, J.-Y. Influence of Pre-Freezing Temperature on the Corneal Endothelial Cytocompatibility and Cell Delivery Performance of Porous Hyaluronic Acid Hydrogel Carriers. Int. J. Mol. Sci. 2015, 16, 18796-18811.

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