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Int. J. Mol. Sci. 2015, 16(6), 13815-13828; doi:10.3390/ijms160613815

Regulation of Human Trophoblast GLUT3 Glucose Transporter by Mammalian Target of Rapamycin Signaling

Laboratory of Reproductive Endocrinology, Department of Physiology, Harbin Medical University, Harbin 150081, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Philip Newton Baker
Received: 5 April 2015 / Accepted: 9 June 2015 / Published: 16 June 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Glucose transporter isoform-3 (GLUT3), one of the primary placental facilitative glucose transporters responsible for basal glucose transport, has a crucial role in glucose transport and fetal growth during early pregnancy. A GLUT3 mutation in mice has been reported to cause loss of early pregnancy or late-gestational fetal growth restriction. However, the underlying mechanisms that regulate the placental GLUT3 transporter in humans are largely unknown. In the present study, we used the JEG-3 human choriocarcinoma cell line, which resembles a first trimester placental model, to study the role of the mammalian target of rapamycin complex 1 (mTORC1) in the regulation of placental GLUT3. We combined rapamycin treatment and small interfering (si) RNA-mediated silencing approaches with mRNA and protein expression/localization studies to investigate the alteration of GLUT3 expression and localization following mTORC1 inhibition in JEG-3 trophoblasts. Inhibition of mTORC1 signaling by silencing raptor decreased GLUT3 mRNA expression (−41%) and protein expression (−50%). Similar effects were obtained in cells in which mTORC1 was inhibited by rapamycin. Immunofluorescence analysis revealed that GLUT3 expression was markedly reduced in the cell surface and cytoplasm of JEG-3 cells in response to mTORC1 silencing. Because placental mTORC1 activity and GLUT3 expression are decreased in human intrauterine growth restriction, our data suggested one possible mechanism for the abnormal fetal growth in this pregnancy complication. View Full-Text
Keywords: placenta; glucose transporter isofrom-3; mammalian target of rapamycin placenta; glucose transporter isofrom-3; mammalian target of rapamycin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Xu, J.; Lu, C.; Wang, J.; Zhang, R.; Qian, X.; Zhu, H. Regulation of Human Trophoblast GLUT3 Glucose Transporter by Mammalian Target of Rapamycin Signaling. Int. J. Mol. Sci. 2015, 16, 13815-13828.

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