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Int. J. Mol. Sci. 2015, 16(4), 7707-7722; doi:10.3390/ijms16047707

Combined Enzymatic and Mechanical Cell Disruption and Lipid Extraction of Green Alga Neochloris oleoabundans

College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China
Author to whom correspondence should be addressed.
Academic Editor: Claire Hellio
Received: 27 February 2015 / Revised: 17 March 2015 / Accepted: 27 March 2015 / Published: 7 April 2015
(This article belongs to the Special Issue Microalgal Biotechnology)
View Full-Text   |   Download PDF [3416 KB, uploaded 7 April 2015]   |  


Microalgal biodiesel is one of the most promising renewable fuels. The wet technique for lipids extraction has advantages over the dry method, such as energy-saving and shorter procedure. The cell disruption is a key factor in wet oil extraction to facilitate the intracellular oil release. Ultrasonication, high-pressure homogenization, enzymatic hydrolysis and the combination of enzymatic hydrolysis with high-pressure homogenization and ultrasonication were employed in this study to disrupt the cells of the microalga Neochloris oleoabundans. The cell disruption degree was investigated. The cell morphology before and after disruption was assessed with scanning and transmission electron microscopy. The energy requirements and the operation cost for wet cell disruption were also estimated. The highest disruption degree, up to 95.41%, assessed by accounting method was achieved by the combination of enzymatic hydrolysis and high-pressure homogenization. A lipid recovery of 92.6% was also obtained by the combined process. The combined process was found to be more efficient and economical compared with the individual process. View Full-Text
Keywords: microalga; cell disruption; ultrasonication; high pressure homogenization; enzymatic lyses microalga; cell disruption; ultrasonication; high pressure homogenization; enzymatic lyses

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, D.; Li, Y.; Hu, X.; Su, W.; Zhong, M. Combined Enzymatic and Mechanical Cell Disruption and Lipid Extraction of Green Alga Neochloris oleoabundans. Int. J. Mol. Sci. 2015, 16, 7707-7722.

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